福尔马林固定石蜡包埋组织切片蛋白质组分析方法与应用研究进展

Advances in Proteomics Techniques and Applications of Formalin-fixed and Paraffin-embedded Tissue Sections

福尔马林固定石蜡包埋(Formalin-fixed and paraffin-embedding,FFPE)是最常见的临床组织保存技术,FFPE组织具有标准化的制备流程、易于存储、标本量大、包含较完整的临床回顾性信息等特点,而成为疾病生物标志物发掘的良好载体。近年来,基于临床FFPE组织的特点,发展了系列蛋白质组学研究方法,包括样本制备、消解、分离到蛋白质质谱鉴定等多个领域,总体呈现出微量、高灵敏度、高通量的技术特点,并已成功用于肿瘤精准医学等临床蛋白质组研究。该综述将对FFPE组织切片样本的蛋白质组学方法,以及其在肿瘤研究中的应用进行概述,从而为临床精准医学蛋白质组学的研究提供借鉴思路。

Formalin-fixed and paraffin-embedding(FFPE)is the most commonly used technique for clinical tissue preservation,while FFPE tissues have the characteristics including standardized preparation process,easy to store,large sample quantity and retrospective clinical information,which make them the high quality library for biomarker discovery and verification.In recent years,a series of proteomics approaches have been developed,covering sample preparation,protein digesting,peptide separation and identification by mass spectrometry.The whole processes have the characteristics of micro-scale sample quality,high sensitivity and high throughput.These methods have been successfully used in clinical proteomics researches,for example,the tumor precision medicine.The proteomics methods for FFPE tissue samples and their applications in oncobioloby are summarizd in this review,providing a valuable reference for proteomics based clinical precision medicine researches.