羰基化蛋白质组学分析进展

Progress in Proteomic Analysis of Protein Carbonylation

蛋白质羰基化作为一种不可逆的翻译后修饰,与诸多疾病和衰老密切相关。有关蛋白质羰基化的各项研究受制于其低丰度、低电离效率及化学相对不稳定性而发展较慢。基于质谱的蛋白质组学分析技术的进步,使蛋白质羰基化的规模化研究成为可能,进而为蛋白质羰基化的相关调控通路研究提供了数据支撑。该综述介绍了蛋白质羰基化的概念、途径、检测方式,并重点介绍了蛋白组学技术应用于蛋白质羰基化分析的进展。

Protein carbonylation,an irreversible post-translational modification,is closely related to many diseases and aging.The analysis of protein carbonylation is a challenge due to its low abundance,low ionization efficiency and relative chemical instability.Advances in mass spectrometry-based proteomics have made it possible to conduct large-scale studies on protein carbonylation,providing in turn the data support for the study of related regulatory pathways of protein carbonylation.The concept,pathways and analysis methods of protein carbonylation are presented in this review,and the advances in proteomic analysis of protein carbonylation are highlighted.