盖印法加工纸基微流控芯片及其应用

Preparation of Paper-based Microfluidic Chips Processed by Imprinted Method and Their Application

建立了一种盖印法加工纸基微流控芯片的方法。滤纸经烷基烯酮二聚体(AKD)的正己烷溶液浸泡,以三乙醇胺溶液为阻断试剂,用绘图软件设计芯片图案并制作光敏印章,用带有芯片图案的印章将阻断试剂盖印在滤纸表面。滤纸在105℃烘箱中加热10 min后,非盖印区域呈疏水性,盖印区域呈亲水性,从而形成液体流动通道。考察了疏水试剂浓度、阻断试剂的选择及配比、盖印分辨率等因素对纸芯片性能的影响。制得的纸芯片用于亚硝酸钠溶液的比色分析,获得良好的线性关系。该方法具有成本低廉、操作简便快速等特点,在制备廉价易普及的纸芯片方面有一定应用前景。

A new method of imprinting lithography for processing paper-based microfluidic chip was developed in this paper.The filter paper used in the study was soaked in the hexane solution containing alkylketone dimmer(AKD),and the triethanolamine solution was used as the blocking reagent.Painter software was applied to design chip pattern and make photosensitive stamp,which was then printed on the surface of the filter paper in accordance with the designed pattern.After the filter paper was heated in 105 ℃ oven for over 10 min,the non-imprinted area was hydrophobic,and the imprinted area was hydrophilic.Thus,a liquid flow channel was formed.Influences of concentration of hydrophobic agent,selection and proportion of the blocking reagent and imprinted resolution on the property of paper-based microfluidic chips were investigated.The prepared paper-based microfluidic chips were used in colorimetric analysis of sodium nitrite solution,and a good linear relationship was obtained.Comprehensive experimental analysis results showed that,with low cost,simple operation and fast analysis,the method has a wide application prospect in production of inexpensive and easily-popularized paper chips.