黄金芽遮阴及返黄的生理生化特性研究

Study on the Physiological and Biochemical Characteristics of Shading and Yellowing of Golden Bud

为探究不同光照强度对黄金芽遮阴及返黄生理生化特性的影响,通过遮阴处理,对黄金芽遮阴前和遮阴后一芽二叶叶片的色素物质、叶绿体结构和生化成分进行分析。结果表明,叶绿体内的结构差异、基粒片层的发达程度与叶绿素含量变化一致,表明叶绿体结构影响色素含量与黄化过程。遮阴后,叶绿素a、叶绿素b含量极显著增加(p<0.01),其中处理A叶绿素a增加了8.71倍;整个试验过程氨基酸含量减少,茶多酚、可溶性糖含量均增加,处理A和处理C黄酮含量变化显著增加,水浸出物含量无明显变化。“黄金芽”叶色变异的遮阴时间在两个月以上,返黄时间一个月以上。

In order to explore the effect of different light intensity on the physiological and biochemical characteristics of shading and yellowing of Golden Bud,the pigment substances,chloroplast structure and biochemical components of the first and second leaves of Golden Bud before and after shading treatment were analyzed.The results showed that the structural differences in chloroplasts and the development of basal lamellae were consistent with the change of chlorophyll content,which indicated that chloroplast structure affected pigment content and yellowing processes.After shading,the content of chlorophyll a and b increased significantly(p<0.01),of which chlorophyll a in treatment A increased by 8.71 times,the amino acid content decreased during the whole experiment,the contents of tea polyphenols and soluble sugars increased,and the content of flavonoids in treatment A and C increased significantly,and the content of water extracts did not change significantly.The shading time of leaf color variation of“Golden Bud”was more than two months,and the time of yellowing was more than one month.