骨髓间充质干细胞对肝星状细胞的接触调节作用

Contact regulation of bone marrow mesenchymal stem cells on hepatic stellate cells

目的探讨骨髓间充质干细胞(BMSC)对肝星状细胞(HSC)的直接接触调节作用。方法建立HSC及BMSC共培养系(共培养组),共培养72 h后通过流式细胞术分离细胞,采用Western blot分别检测共培养组HSC中Notch通路相关受体(Notch1、Notch2、Notch3及Notch4)及BMSC中Notch相关配体(Dll1,Dll3,Dll4,Jag1及Jag2)的表达情况,以单独培养HSC及BMSC为对照组。两组蛋白表达比较采用t检验。结果共培养组中Notch1表达量为1.85±0.34,明显高于对照组的0.98±0.25(t=4.235,P<0.05);而Notch3表达为0.40±0.12,明显低于对照组的1.00±0.08(t=-7.971,P<0.05);Notch相关配体Jag1表达为2.03±0.30,明显高于对照组的0.99±0.13(t=6.386,P<0.05)。结论BMSC可能通过Jag1结合Noch1以直接接触机制调控HSC。

Objective To investigate the direct contact regulation of bone marrow mesenchymal stem cells(BMSCs)upon hepatic stellate cells(HSCs).Methods The co-culture system of HSC and BMSC(co-culture group)was established.After 72-h co-culture,the cells were isolated by flow cytometry.The expression levels of related receptors of Notch signaling pathway(Notch1,Notch2,Notch3 and Notch4)in HSCs and Notch-related ligands(DII1,DII3,DII4,Jag1 and Jag2)in BMSCs from the co-culture group were detected by Western blot.HSCs and BMSCs which were cultured separately were taken as the control group.The expression levels of proteins were statistically compared between two groups using t test.Results In the co-culture group,the expression level of Notch1 was 1.85±0.34,significantly higher than 0.98±0.25 in the control group(t=4.235,P<0.05).However,the expression of Notch3 was 0.40±0.12,significantly lower than 1.00±0.08 in the control group(t=-7.971,P<0.05).The expression level of Notch-related ligand Jag1 was 2.03±0.30,significantly higher compared with 0.99±0.13 in the control group(t=6.386,P<0.05).Conclusion BMSCs may regulate HSCs through combining Jag1 with Noch1 in a direct contact way.