uc003yoa.3与肝癌肝切除术后患者预后的关系

Relationship between uc003yoa.3 and clinical prognosis of patients with hepatocellular carcinoma after hepatectomy

目的探讨翻译起始因子uc003yoa.3对肝细胞癌(肝癌)肝切除术后生存的影响及其在肝癌细胞增殖中的作用。方法回顾性分析2010年3月至4月东方肝胆外科医院行肝切除的215例患者临床资料。患者均签署知情同意书,符合医学伦理学规定。其中男182例,女33例;年龄30~76岁,中位年龄49岁。采用基因芯片分析5例患者肝癌及配对癌旁组织uc003yoa.3 mRNA的表达。RT-PCR检测siRNA干扰后SMMC7721肝癌细胞uc003yoa.3 mRNA表达,CCK-8检测细胞增殖。多组uc003yoa.3 mRNA比较采用单因素分析和LSD-t检验。采用Kaplan-Meier法绘制生存曲线,Log-rank检验进行生存分析,Cox比例风险模型分析生存预后独立危险因素。结果基因芯片检测显示肝癌组织样本中uc003yoa.3 mRNA表达明显高于癌旁组织(Fold change=3.654,P<0.05)。uc003yoa.3-siRNA作用SMMC7721肝癌细胞4 d后,uc003yoa.3 mRNA表达量为0.14±0.04,明显低于对照组的0.22±0.06(LSD-t=-1.119,P<0.05);SMMC7721增殖抑制率52%。Cox多因素分析显示,uc003yoa.3高表达、AFP>20μg/L、肿瘤直径>5 cm、卫星灶是影响肝癌患者无瘤生存的独立危险因素(HR=1.979,1.841,2.539,2.358;P<0.05)。uc003yoa.3高表达及肿瘤直径>5 cm是影响肝癌患者肝切除术后总体生存的独立危险因素(HR=1.927,2.134;P<0.05)。结论uc003yoa.3在肝癌中高表达,可促进肝癌细胞恶性表型增加,且是影响肝癌肝切除术后长期生存的独立危险因素。

Objective To investigate the effect of translation initiation factor uc003yoa.3 on the survival of patients with hepatocellular carcinoma(HCC)after hepatectomy and its role in the proliferation of HCC cells.Methods Clinical data of 215 patients who underwent hepatectomy in Eastern Hepatobiliary Surgery Hospital from March to April 2010 were retrospectively analyzed.The informed consents of all patients were obtained and the local ethical committee approval was received.Among them,182 patients were male and 33 female,aged 30-76 years with a median age of 49 years.The expression level of uc003yoa.3 mRNA of 5 patients'HCC and matched para-cancerous tissues was analyzed by gene chip.The expression of uc003yoa.3 mRNA in SMMC7721 cells after siRNA interference was measured by RTPCR.Cell proliferation was detected by CCK-8.The uc003yoa.3 mRNA was statistically compared among different groups by univariate analysis and LSD-t test.The survival curve was delineated by Kaplan-Meier method.Survival analysis was performed by Log-rank test.The independent risk factors for the survival were identified by Cox's proportional hazards model.Results Gene chip analysis demonstrated that the expression of uc003yoa.3 mRNA in the HCC tissues was significantly higher than that in the matched paracancerous tissues(Fold change=3.654,P<0.05).After SMMC7721 cells were treated with uc003yoa.3-siRNA for 4 d,the expression of uc003yoa.3 mRNA was 0.14±0.04,significantly lower than 0.22±0.06 in the control group(LSD-t=-1.119,P<0.05).The proliferation inhibition rate of SMMC7721 was 52%.Cox's multivariate analysis showed that high expression of uc003yoa.3,AFP>20μg/L,tumor diameter>5 cm and satellite lesion were the independent risk factors for tumor-free survival of HCC patients(HR=1.979,1.841,2.539,2.358;P<0.05).High expression of uc003yoa.3 and tumor diameter>5 cm were the independent risk factors for the overall survival of HCC patients after hepatectomy(HR=1.927,2.134;P<0.05).Conclusions High expression of uc003yoa.3 in HCC patients can promote the increase of malignant phenotype of HCC cells,which is an independent risk factor for the long-term survival of HCC patients after hepatectomy.