液相色谱法测定肉制品中万古霉素的残留量

Determination of Vancomycin Residues in Meat Products by High Performance Liquid Chromatography

采用液相色谱法测定肉制品中万古霉素的残留量。样品经过0.1%甲酸水-乙腈(体积比为7∶3)提取,二氯甲烷去脂,固相萃取柱净化,利用液相色谱仪,采用外标法定量检测。结果显示,万古霉素液相色谱检测方法的线性范围是8.5μg/mL^170μg/mL,线性相关系数大于0.99。方法检出限为0.5 mg/kg,定量限为8 mg/kg。添加回收率在75%~95%,精密度为5%~8%。该方法适用于肉制品中万古霉素残留量的测定。

The vancomycin residues in meat products were determined by liquid chromatography.The samples were extracted by 0.1%formic acid water-acetonitrile(volume ratio 7∶3)and degreased by dichloromethane.The samples were purified by solid phase extraction column and quantitatively detected by liquid chromatography and external standard method.The results showed that the linear range of vancomycin detection by liquid chromatography was 8.5μg/mL-170μg/mL,and the linear correlation coefficient was more than 0.99.The detection limit was 0.5 mg/kg and the quantitative limit was 8 mg/kg.The recovery and precision are 75%-95%and 5%-8%respectively.This method was suitable for the determination of vancomycin residues in meat products.