汉黄芩素对鼻咽癌CNE2细胞增殖及凋亡的影响

Effect of Wogonin on proliferation and apoptosis of nasopharyngeal carcinoma CNE2 cells

目的探讨汉黄芩素对鼻咽癌CNE2细胞增殖和凋亡的影响及其可能的作用机制。方法采用不同浓度(2.5μmol/L、5.0μmol/L、10.0μmol/L、20.0μmol/L)汉黄芩素作用鼻咽癌CNE2细胞,以溶剂为对照组。采用实时无标记细胞功能分析仪监测细胞增殖情况,双荧光法检测细胞凋亡情况,Western blot法检测PCNA、Survivin、XIAP、Bcl-2蛋白表达水平。结果不同浓度(2.5μmol/L、5.0μmol/L、10.0μmol/L、20.0μmol/L)汉黄芩素作用24 h后CNE2细胞增殖抑制率依次为20.3%、23.7%、33.4%、40.9%,IC50为42.41μmol/L;36 h依次为18.0%、22.0%、36.1%、40.5%,IC50为34.46μmol/L;48 h依次为7.4%、20.2%、35.0%、40.9%,IC50为22.81μmol/L。与溶剂对照组比较,10.0μmol/L、20.0μmol/L汉黄芩素组细胞凋亡率均升高(t=23.710,P=0.001;t=43.934,P<0.001)。20.0μmol/L汉黄芩素处理鼻咽癌CNE2细胞48 h后,与溶剂对照组比较,Bax蛋白表达水平上升(P<0.05),Survivin、XIAP、Bcl-2蛋白表达水平下降(P<0.05)。结论汉黄芩素可抑制鼻咽癌CNE2细胞增殖并诱导其凋亡,可能通过促进Bax蛋白表达并抑制Survivin、XIAP、Bcl-2蛋白表达发挥作用。

Objective To investigate the effect of Wogonin on the proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE2 and the related possible mechanism. Methods CNE2 cells were treated with Wogonin at different concentrations(2.5 μmol/L,5.0 μmol/L,10.0 μmol/L,and 20.0 μmol/L),with the solvent as the control group. The real-time unlabeled cell function analysis technique was used to monitor the proliferation of CNE2 cells,and the double fluorescent labeling method was used to detect the apoptosis of CNE2 cells,and Western blot was used to detect the protein expression of PCNA,Survivin,XIAP,and Bcl-2. Results Wogonin could inhibit the proliferation of CNE2 cells at different concentrations(2.5 μmol/L,5.0 μmol/L,10.0 μmol/L,20.0 μmol/L),and when treated for 24 h,the inhibition ratios were 20.3%,23.7%,33.4%,and 40.9%,respectively,and the IC50 was 42.41 μmol/L;when treated for 36 h,the inhibition ratios were 18.0%,22.0%,36.1%,and 40.5%,respectively,and the IC50 was 34.46 μmol/L;when treated for 48 h,the inhibition ratios were 7.4%,20.2%,35.0%,and 40.9%,respectively,and the IC50 was 22.81 μmol/L. Compared to those of the solvent control group,the apoptosis rates of CNE2 cells treated with Wogonin(10 μmol/L,20 μmol/L)increased(t=23.710,P=0.001;t=43.934,P<0.001). When CNE2 cells were treated with 20 μmol/L Wogonin for 48 hours,the expression of Bax protein increased significantly(P<0.005),whereas the expressions of Survivin,XIAP,and Bcl-2 proteins decreased(P<0.005). Conclusion Wogonin can inhibit the proliferation and induce the apoptosis of nasopharyngeal carcinoma CNE2 cells,probably through promoting the expression of Bax protein while inhibiting the expressions of Survivin,XIAP,and Bcl-2 proteins.