高半胱氨酸-罗丹明探针可视化检测水溶液中Hg^2+

Visual detection for Hg^2+ in aqueous solution with Homocysteine-Rhodamine probe

基于硫原子的亲汞性,通过引入高半胱氨酸(Hcy)为识别基团,制备了一种新型的Hg^2+荧光探针Hcy-Rh6G2,并命名为2-(((3,6-二乙氨基-2,7-二甲基-3-氧螺环[异吲哚-1,9-咕口屯]-2-酰基)-亚氨基)亚乙基胺)-4-巯基丁酸.考察了探针Hcy-Rh6G2的紫外-可见吸收光谱及其对常见离子的选择性测试.结果表明:在HEPES缓冲溶液中,高半胱氨酸中的硫基与Hg^2+结合,会发生脱硫水解反应,使溶液由无色变成粉红色,实现了可视化检测,该识别受常见金属阳离子和阴离子的干扰较小,对Hg^2+的识别过程不可逆,当CH 3OH/HEPES的体积比在1∶1时,紫外吸收强度已经接近最大.能运用于环境中高灵敏,高选择性地检测Hg^2+.

Based on the amphotericity of sulfur atom,we introduced a novel Hg^2+fluorescent probe Hcy-Rh6G2 by introducing homocysteine(Hcy)as a recognition group,and named it 2-((3,6-Diethylamino-2,7-dimethyl-3-oxospiro[isoindol-1,9-fluorenyl]-2-yl)-imino)ethylideneamine-4-mercaptobutyric acid.The UV-visible absorption spectrum of the probe Hcy-Rh6G2 and its selectivity test for common ions were investigated.The results show that in the HEPES buffer solution,the sulfur group in homocysteine binds to Hg^2+,and the desulfurization and hydrolysis reaction occurs,which makes the solution change from colorless to pink,which realizes visual detection,which is recognized by common metal cations and The interference of anions is small,and the recognition process of Hg^2+is irreversible.When the volume ratio of CH 3OH/HEPES is 1∶1,the UV absorption intensity is close to the maximum.It can be used in the environment to detect Hg^2+with high sensitivity and high selectivity.