G蛋白偶联受体35在胃癌中的表达及其与预后的相关性

Expression and prognostic value of GPR35 in gastric cancer

目的挖掘分析G蛋白偶联受体35(GPR35)基因在胃癌中的表达及意义。方法采用cBioportal分析工具对TCGA数据库中GPR35在胃癌组织的基因改变频率进行分析;运用Oncomine数据库分析胃癌组织与正常胃黏膜中GPR35 mRNA表达的差异性;应用SPSS软件分析GPR35在胃癌组织中的表达水平与患者临床病理参数之间的关系;运用Kaplan-Meier Plotter数据库探讨GPR35 mRNA表达水平与胃癌患者预后的关系。结果在TCGA数据库中GPR35基因改变形式主要表现为缺失。除了Oncomine数据库DErrico数据集中GPR35 mRNA在混合型胃癌中的表达较正常胃黏膜降低(P=0.501),在不同分型(肠型、弥漫型和混合型)胃癌中GPR35 mRNA的表达均高于正常胃黏膜(P<0.05)。GPR35的表达与肿瘤的分化程度、TNM分期、淋巴结转移和远处转移以及HER2表达与否显著相关,而与患者性别、肿瘤浸润深度、Lauren分型和治疗策略均无显著相关性。通过Kaplan-Meier Plotter数据库分析发现,GPR35 mRNA高表达的胃癌患者总生存期[HR=1.62(1.36~1.93),P=3.6×10^-8],首次进展[HR=1.52(1.24~1.86),P=4.2×10^-5]及进展后生存期[HR=2.53(2.02~3.16),P<1×10^-16]均缩短,患者预后差。结论GPR35 mRNA在胃癌中的表达是上调的,其表达水平与胃癌患者的生存期显著相关,提示GPR35可能作为判断胃癌患者预后的潜在生物学指标以及分子靶向治疗的靶点。

Objective To analysis the expression and prognostic value of GPR35 in the tissue of gastric cancer.Methods The genomic alternation of GPR35 was performed using cBioportal tool,which is based on TCGA database.The difference in mRNA expression of GPR35 between gastric cancer tissues and normal gastric mucosa was analyzed according to Oncomine database.We explored the correlation between expression of GPR35 and clinicopathological characteristics of patients with gastric cancer by SPSS software.The association between GPR35 and patients with gastric cancer was demonstrated by Kaplan-Meier plotter database.Results The most frequent change of GPR35 gene was deletion in TCGA database.Except for higher expression of GPR35 in mixed gastric cancer analyzed in DErrico gastric statistics of Oncomine database(P=0.501),the expression of GPR35 at mRNA levels in all Lauren type of gastric cancer was higher than in normal gastric mucosa(P<0.05).GPR35 expression was significantly correlated with tumor differentiation,TNM stage,lymph node metastasis,distant metastasis,and the expression of HER2,but there was no significant correlation between the expression of GPR35 and gender,depth of invasion,Lauren typing and treatment strategy.The patients with high expression of GPR35 had shorter the overall survival time[HR=1.62(1.36-1.93),P=3.6×10^-8],the post progression survival[HR=1.52(1.24-1.86),P=4.2×10^-5]and the first progression[HR=2.53(2.02-3.16),P<1×10^-16].Conclusion The upregulation of GPR35 is observed in gastric cancer.GPR35 may be a potential biological indicator to determine the prognosis of gastric cancer patients and a target for molecular targeted therapy.