补肾益精丸对雷公藤多苷诱导睾丸支持细胞损伤的影响

The Influence of Kidney-Nourishing and Essence-Boosting Pill on Injury of Sertoli Cells Induced by Tripterygium Wilfordii Glycosides

目的:探讨补肾益精丸干预雷公藤多苷诱导睾丸支持细胞损伤的作用机制。方法:将36只SD大鼠按体质量随机分为无药血清组、雷公藤多苷组和补肾益精丸组,每组各12只,制备含药血清。体外培养小鼠睾丸支持细胞,取对数生长期细胞分为空白对照组、无药血清组、雷公藤多苷组和补肾益精丸组,每组4孔。血清干预后采用MTT法测定细胞增殖率,流式细胞仪测定细胞周期。结果:补肾益精丸组细胞增殖率高于雷公藤多苷组,差异具有统计学意义(P<0.05),补肾益精丸组细胞增殖率与空白对照组比较,差异无统计学意义(P>0.05)。与空白对照组比较,无药血清组及雷公藤多苷组G1期细胞数增多,S期细胞数减少;补肾益精丸组与雷公藤多苷组比较,S期细胞数增多。结论:雷公藤多苷对睾丸支持细胞的增殖具有抑制作用,主要作用于细胞G1期及S期。补肾益精丸可有效拮抗雷公藤多苷对睾丸支持细胞的损伤。

Objective:To explore the mechanism of action of Kidney-Nourishing and Essence-Boosting Pill(KNEBP)on injury of sertoli cells induced by tripterygium wilfordii glycosides.Methods:Thirty six SD rats were randomly divided into three groups according to their body weight:the no drug serum group,the tripterygium wilfordii polyglycoside group and the group of KNEBP,with 12 cases in each group.The drug containing serum was prepared.The sertoli cells of mouse testis were cultured in vitro and logarithmic growth phase cells were taken and then divided into four groups:the blank control group,the no drug serum group,tripterygium glycoside group and the group of KNEBP,with 4 holes in each group.Cell proliferation rate was measured by MTT method and cell cycle was measured by flow cytometry after serum intervention.Results:The cell proliferation rate of the group of KNEBP was higher than that of tripterygium wilfordii polyglycoside group,the difference was statistically significant(P<0.05).There was no significant difference between the group of KNEBP and the blank control group(P>0.05).Compared with the blank control group,the number of G1 phase cells increased and that of S phase cells decreased in the no drug serum group and the tripterygium glycosides group.Compared with the tripterygium wilfordii polyglycosides group,the number of S phase cells of the group of KNEBP increased.Conclusion:Tripterygium wilfordii glycoside can inhibit the proliferation of sertoli cells mainly in G1 and S phases.Kidney-Nourishing and Essence-Boosting Pill can effectively antagonize the damage of tripterygium wilfordii glycoside on sertoli cells.