摘要
【目的】盐碱地的形成,不仅造成了资源浪费,也严重制约着农业生产。藜麦具有耐盐的特性,能够缓解部分盐胁迫。中国是稀土含量最多的国家,有研究表明,稀土元素镧可能对盐分胁迫有缓解作用。本试验通过硝酸镧浸种后对藜麦幼苗进行盐胁迫处理,探讨该浸种方法对藜麦种子萌发以及盐胁迫下幼苗生长的影响,为缓解盐害、提高藜麦抗盐性研究提理论依据。【方法】本研究以藜麦为研究材料,采用温室盆栽法,研究不同硝酸镧浓度浸种(25、50和100 mg·L^-1)对藜麦种子萌发及不同浓度盐胁迫下(100、200和300 mmol·L^-1 NaCl溶液)对幼苗生长的影响。【结果】(1)50 mg·L^-1 La(NO3)3浸种时,藜麦种子的萌发效果最好,发芽率、发芽势和发芽指数均达到最高,并与其他浓度处理具有显著差异;(2)相同La(NO3)3浓度浸种时,300 mmol·L^-1NaCl以内藜麦幼苗的株高、根长随着盐胁迫浓度的增加逐渐降低,POD、SOD、MDA、可溶性糖、脯氨酸都随着盐胁迫浓度的增加而升高;(3)在相同浓度盐胁迫下,藜麦幼苗的株高、根长,POD、SOD、可溶性糖、可溶性蛋白、脯氨酸均随硝酸镧浸种浓度的增加呈先升后降的变化趋势,MDA随浸种浓度的增加呈先降后升的变化趋势;(4)藜麦在300 mmol·L^-1NaCl范围下均能生长,在300 mmol·L^-1 NaCl时,幼苗各项指标基本最好,其中50 mg·L^-1硝酸镧浸种处理下各项生长指标均达到最佳。【结论】低浓度50 mg·L^-1硝酸镧能够促进藜麦种子的萌发以及盐胁迫下藜麦幼苗地上部分生长,加强藜麦幼苗的抗氧化酶活性,增加渗透调节物质含量,对盐胁迫起到缓解作用;高浓度则抑制生长。本文结果表明硝酸镧浸种可提高藜麦对盐分胁迫的抗性。
【Objective】With the formation of soil salinization,not only causes the resource-wasting,but also restricts the agricultural production.Quinoa has salt-tolerant properties,It can alleviate salt stress.China is the country with the most rare earth content.There is a study that lanthanum may alleviate the effects of salt stress on plants.In this study,quinoa was treated with salt stress,which seed had been soaked with lanthanum nitrate before.The effects of soaking seeds with lanthanum nitrate on seed germination and seedling growth of quinoa under salt stress were examined to find a way to improve salt resistance of the species.【Method】In this study,quinoa was used as the research material and greenhouse potted planting method was adopted in order to study the effects of different lanthanum nitrate leaching species(25,50,100 mg·L^-1)on seed germination and seedling growth under different salt stresses(100,200,300 mmol·L^-1 sodium chloride solution).【Result】(1)When lanthanum nitrate was 50 mg·L^-1,the effect of quinoa seeds were the optimal,the germination percentage,germination potential,and germination index of quinoa seeds were the highest,and there were significant differences compared with other concentrations.(2)At the same socking concentration,plant height and root length of seedlings decreased with the increase of salt concentrations within 300 mmol·L^-1 NaCl,while peroxidase(POD),superoxide dismutase(SOD),malondialdehyde(MDA),soluble sugar,proline and other physiological and biochemical indexes increased with the increase of salt concentrations.(3)At the same salt concentration,plant height,root length and other growth indicators of quinoa seedlings showed the tendency of first increasing and then decreasing with the increase of soaking concentrations,as well as POD,SOD,soluble sugar,soluble protein,proline and other physiological and biochemical indexes.For MDA,the trends were reversed.(4)Quinoa seedlings survived and grown in the NaCl solutions less than 300 mmol·L^-1,but optimal concentration was 300 mmol·L^-1.At the same time 300 mmol·L^-1 salt concentration,the growth index were the best when lanthanum nitrate was 50 mg·L^-1.【Conclusion】Under salt stress,quinoa seeds socked in low concentration solution of lanthanum nitrate could promote the seed germination and the shoot growth,strengthen the antioxidant enzyme activities of seedlings,and improve the content of the osmotic adjustment material,resulting in increasing resistance to salt stress.However,seedling growth was inhabited by high concentration solution of lanthanum nitrate.This study suggested that the resistance of quinoa to salt stress was enhanced by adding adequate lanthanum nitrate.
作者
庞春花
张媛
李亚妮
PANG ChunHua;ZHANG Yuan;LI YaNi(Shool of Life Science,Shanxi Normal University,Linfen 041004,Shanxi;Collaborative Innovation Center for Shanxi Advanced Permanent Magnetic Materials and Technology,Linfen 041004,Shanxi)
出处
《中国农业科学》
CAS
CSCD
北大核心
2019年第24期4484-4492,共9页
Scientia Agricultura Sinica
基金
国家自然科学基金(31571604)
山西省先进永磁材料与技术协同创新项目(030100030022)
关键词
藜麦
种子萌发
幼苗
硝酸镧
盐胁迫
quinoa
seed germination
seedling
lanthanum nitrate
salt stress