牛磺酸对LPS诱导小鼠急性肺损伤的治疗效果

Therapeutic Effect of Taurine on LPS-induced Acute Lung Injury in Mice

目的评价牛磺酸对LPS诱导急性肺损伤小鼠的治疗作用。方法将BALB/c小鼠随机分为正常对照组、LPS模型组、牛磺酸低、中、高剂量组,正常对照组小鼠经鼻吸入50μl生理盐水,LPS模型组小鼠经鼻吸入8 mg/ml的LPS溶液50μl,牛磺酸低、中、高剂量组分别经腹腔注射100、200、400 mg/kg牛磺酸,监测并比较5组小鼠肺功能、肺指数及髓过氧化物(MPO)酶活性。结果①LPS模型组呼气末期停顿(EEP)、最大呼气流量(PEF)、支气管收缩的程度(PENH)、吸气时间(TI)及呼气时间(TE)均高于正常对照组及牛磺酸低、中、高剂量组;牛磺酸低、中、高剂量组EEP、PEF、PENH、TI及TE均高于正常对照组,且依次降低,差异有统计学意义(P<0.05)。LPS模型组呼吸频率(RR)、呼吸松弛时间(RT)、每分钟呼气量(MV)、吸气末期停顿时间(EIP)、最大吸气流量(PIF)、潮气量(TV)及呼气量(EV)均低于正常对照组及牛磺酸低、中、高剂量组;牛磺酸低、中、高剂量组RR、RT、MV、EIP、PIF、TV及EV均低于正常对照组,且依次升高,差异有统计学意义(P<0.05)。②LPS模型组肺湿/干重比、MPO酶活性高于正常对照组及牛磺酸低、中、高剂量组;牛磺酸低、中、高剂量组肺湿/干重比、MPO酶活性高于正常对照组,且依次降低,差异有统计学意义(P<0.05)。结论牛磺酸对LPS诱导的小鼠急性肺损伤具有一定的保护作用,可减轻小鼠肺损伤,且其呼吸功能改善程度与剂量有一定联系。

Objective To evaluate the therapeutic effect of taurine on LPS-induced acute lung injury in mice.Methods BALB/c mice were randomly divided into normal control group,LPS model group,taurine low,medium and high dose groups.Normal control group mice inhaled 50μl physiological saline,mice in the LPS model group inhaled 50μl of 8 mg/ml LPS solution nasally,and the low,medium,and high dose groups of taurine were injected intraperitoneally with 100,200,and 400 mg/kg taurine.Rat lung function,lung index,and myeloperoxidase(MPO)enzyme activity.Results①The end-expiratory pause(EEP),the maximum expiratory flow(PEF),the degree of bronchoconstriction(PENH),the inspiratory time(TI)and the expiratory time(TE)in the LPS model group were higher than those in the normal control group and taurine.The low,medium,and high dose groups;the low,medium,and high dose taurine EEP,PEF,PENH,TI,and TE were all higher than the normal control group,and they decreased in order,the difference was statistically significant(P<0.05).LPS model group breathing frequency(RR),breathing relaxation time(RT),expiratory volume per minute(MV),end of inhalation time(EIP),maximum inspiratory flow(PIF),tidal volume(TV)and exhalation amount(EV)is lower than the normal control group and low,medium and high dose of taurine;RR,RT,MV,EIP,PIF,TV and EV of the low,medium and high dose of taurine are lower than the normal control group,and increased in order,the difference was statistically significant(P<0.05).②The lung wet/dry weight ratio and MPO enzyme activity of the LPS model group were higher than those of the normal control group and low,medium,and high dose taurine;lung wet/dry weight ratio and MPO enzyme activity of the low,medium,and high dose taurine group was higher than the normal control group and decreased in order,the difference was statistically significant(P<0.05).Conclusion Taurine has a certain protective effect on acute lung injury induced by LPS in mice,which can alleviate lung injury in mice,and its degree of improvement in respiratory function is related to the dose.