miR-301b-3p通过靶向SATB2抑制肝癌细胞增殖

miR-301b-3p inhibits cell proliferation by targeting SATB2 in hepatocellular carcinoma

目的研究miR-301b-3p在肝细胞癌中的表达情况及其对肝癌细胞增殖的影响。方法采用qRT-PCR检测miR-301b-3p在肝癌组织和细胞系中的表达情况。采用免疫印迹法和荧光素酶报告子法研究miR-301b-3p与SATB2蛋白的相互作用关系。采用CCK8实验评估细胞的增殖能力。结果在本研究中,qRT-PCR结果证实miR-301b-3p在HCC组织和肝癌细胞系中下调表达。将miR-301b-3p mimic转染至HepG2细胞后,HepG2细胞的增殖能力显著被抑制。结合生物信息学分析、荧光素酶报告实验、qRT-PCR以及免疫印迹实验中证实,SATB2是miR-301b-3p在肝癌细胞中的直接作用靶点。在HepG2细胞中,miR-301b-3p抑制SATB2的mRNA和蛋白表达水平。结论本研究结果表明,miR-301b-3p通过抑制SATB2调控肝癌细胞增殖,提示miR-301b-3p可能是肝癌治疗的一个潜在的靶点。

Objective To study expression of miR-301 b-3 p in hepatocellular carcinoma(HCC)and its effect on(HCC)proliferation.Methods Expression of miR-301 b-3 p in HCC tissues and cell lines was detected by qRT-PCR.A western blot assay and Luciferase reporter assay were employed to study the interaction between miR-301 b-3 p and AT-rich sequence-binding protein-2(SATB2).Cell proliferation was measured by CCK8 analyses.Results In this study it was observed low expression of the miR-301 b-3 p in both HCC tissues and hepatoma cell line as compared to normal control.Further to investigate the role of miR-301 b-3 p in HCC development,HepG2 cells were transfect with miR-301 b-3 p mimic.Following transfection,miR-301 b-3 p expression was significantly increased,which further repressed proliferation of HepG2 cells.Bioinformatics,Luciferase Reporter,and qRT-PCR,and western blotting assays indicated that special SATB2 was a direct target of miR-301 b-3 p in HCC cells.There was a negative correlation between the expression levels of SATB2 and miR-301 b-3 p in HepG2.Conclusion The results of the present study indicated that miR-301 b-3 p regulated HCC cell proliferation through inhibiting SATB2,and suggest that miR-301 b-3 p may serve as a promising target for the treatment of HCC.