Erastin对脂多糖诱导的支气管上皮细胞炎症反应的影响

Effect of erastin on the LPS-induced inflammatory response in human bronchial epithelial cells

目的研究铁死亡激活剂(Erastin)对脂多糖(LPS)诱导的人支气管上皮细胞(HBE)炎症反应产生的作用。方法体外培养HBE细胞,分为3组:⑴PBS对照组:加入等体积PBS;⑵LPS组:加入0.1g/L LPS溶液;⑶ERA组:加入20μmol/L Erastin溶液;⑷LPS+ERA组:加入20μmol/L Erastin溶液预处理30min后,加入0.1g/L LPS溶液;⑸LPS+FER-1组:加入500nmol/L铁死亡抑制剂Ferrostain-1溶液预处理30min后,加入0.1g/L LPS溶液。各组刺激24h后利用荧光定量PCR(qPCR)法检测HBE细胞中IL-6的mRNA相对表达量。结果与PBS对照组相比,LPS组、ERA组和LPS+ERA组IL-6 mRNA相对表达量显著增高(P<0.01);与LPS组相比,LPS+ERA组IL-6 mRNA相对表达量显著增高(P<0.01),LPS+FER-1处理组IL-6 mRNA相对表达量显著降低(P<0.01),差异具有统计学意义。结论Erastin能够诱导支气管上皮细胞释放炎症介质,并能够放大LPS诱导的炎症介质的释放。

Objective To observe the effect of erastin on the LPS-induced inflammatory response in human bronchial epithelial cells.Methods HBE cells were cultured in vitro and divided into 3 groups:⑴PBS contorl group,adding equal volume PBS;⑵LPS group,adding 0.1g/L LPS;⑶ERA group,adding 20umol/L Erastin;⑷LPS+ERA group,adding 20umol/L Erastin,then stimulated with 0.1g/L LPS;⑸LPS+FER-1 group,adding 500nmol/L Ferrostain-1,then stimulated with 0.1g/L LPS.After incubation for 24h,the real-time PCR(qPCR)was performed to examine the mRNA relative expression of IL-6.Results the mRNA relative expression of IL-6 in the LPS group、ERA group and LPS+ERA group was significantly increased compared with the PBS contorl group(all P<0.01);Compared with the LPS group,the mRNA relative expression of IL-6 in the LPS+ERA group was significantly increased(P<0.01),and the LPS+FER-1 group was significantly lower(P<0.01).Conclusion Erastin can amplify LPS-induced human bronchial epithelial cells inflammatory response.