维生素D受体与MCP-1在系统性红斑狼疮患者中的表达及意义

Vitamin D receptor and monocyte chemoattractant protein-1 expressions in peripheral blood mononuclear cells in patients with systemic lupus erythemaotsus

目的观察维生素D受体(VDR)在系统性红斑狼疮患者(SLE)中的表达以及与单核细胞趋化蛋白-1(MCP-1)的关系。方法共计纳入中南湘雅三医院肾脏风湿科住院SLE患者60例和募集28例健康志愿者作为研究对象。使用实时荧光定量聚合链反应方法(QRT-PCR)检测外周血单个核细胞(PBMC)的VDR m RNA和MCP-1 mRNA,Western blot检测VDR蛋白表达,ELISA法检测外周血MCP-1水平,使用单因素方差分析、Pearson相关分析进行统计学分析。结果我们发现SLE患者PBMC的VDR mRNA及蛋白表达量均显著低于正常对照组(P<0.01),活动组低于非活动组(P<0.05);SLE患者PBMC的MCP-1mRNA及血清水平显著高于正常对照组(P<0.01),活动组高于非活动组(P<0.01);Pearson相关分析显示PBMC VDR mRNA与SLE疾病活动指数(SLEDAI)呈负相关(r=-0.417,P=0.001);VDR mRNA与MCP-1 mRNA、VDR蛋白与血清MCP-1浓度分别呈负相关(r=-0.554,P=0.000;r=-0.400,P=0.028)。结论本研究表明SLE患者PBMC的VDR表达下调与SLE疾病活动度成负相关,且可通过影响MCP-1的表达在SLE发病过程中发挥重要作用。

Objective To detect the expressions of vitamin D receptor(VDR) in peripheral blood monocytes(PBMCs) and its association with monocyte chemoattractant protein-1(MCP-1) in patients with systemic lupus erythemaotsus(SLE). Methods We examined the expressions of VDR and MCP-1 mRNAs in the PBMCs in 60 SLE patients and 28 healthy individuals using real-time quantitative PCR. We also detected the expression of VDR protein in the PBMCs using Western blotting and peripheral blood MCP-1 level using ELISA for these participants. The correlation of VDR and MCP-1 expressions with the disease activity index of SLE(SLEDAI) of the patients were analyzed. Results The expressions of VDR mRNA and protein in the PBMCs were significantly lower in patients with SLE than in the healthy individuals(P<0.01), and that in the patients with active disease was lower than in remission(P<0.05). The MCP-1 mRNA expression in the PBMCs and its serum levels were significantly increased in SLE patients as compared with the healthy individuals(P<0.01), and the increase was significantly more obvious in the patients with active disease than in those in remission(P<0.01). Pearson correlation analysis showed that VDR mRNA in the PBMCs was negatively correlated with SLEDAI(r=-0.417, P=0.001);negative correlations were also found between VDR mRNA and MCP-1 m RNA(r=-0.554, P=0.000) and between VDR protein expression and serum MCP-1 level(r=-0.400, P=0.028). Conclusion The down-regulation of VDR expression in the PBMC is negatively correlated with the disease activity of SLE. VDR may play an important role in the pathogenesis of SLE by affecting the expression of MCP-1.