双醋瑞因对眼表常见病原菌的体外抑制作用

In vitroantimicrobial activity of diacerein on common ocular surface pathogens

目的研究双醋瑞因对眼表常见病原菌的体外抑菌作用。方法采集于河南省立眼科医院就诊的眼表感染患者中分离培养的革兰阳性球菌和杆菌、革兰阴性杆菌、丝状真菌及念珠菌。采用K-B琼脂纸片扩散法测定双醋瑞因对病原菌的体外抑菌活性,采用微量液基稀释法进行最低抑菌浓度(MIC)定量测定。以左氧氟沙星和伏立康唑分别为抗细菌药物和抗真菌药物对照。结果双醋瑞因对眼表分离的42株革兰阳性球菌和10株革兰阳性杆菌均有明显抑菌活性,双醋瑞因与左氧氟沙星对表皮葡萄球菌、金黄色葡萄球菌、中间葡萄球菌和革兰阳性棒状杆菌抑菌圈直径比较,差异无统计学意义(均P>0.05)。双醋瑞因对眼表分离的23株革兰阴性杆菌,10株丝状真菌和3株念珠菌均无抑制作用。双醋瑞因对表皮葡萄球菌、金黄色葡萄球菌、中间葡萄球菌及其他葡萄球菌的MIC范围为1~32μg/ml,MIC90分别为16、8、16、32μg/ml。结论双醋瑞因对眼表分离的革兰阳性葡萄球菌和棒状杆菌具有体外抑菌活性,对致病革兰阴性杆菌和真菌无抑菌活性。

Objective To study the antimicrobial activity of diacerein on common pathogens of the ocular surface in vitro.Methods Pathogens were collected from patients with ocular surface infections in Henan Eye Hospital,including Gram-positive cocci and bacilli,Gram-negative bacilli,filamentous fungi,and Candida.The antimicrobial activity of diacerein was determined by the K-B agar diffusion method,and its minimum inhibitory concentration(MIC)was determined by the micro-liquid method.Levofloxacin and voriconazole were used as the control of antibacterial and antifungal drug,respectirely.Results Diacerein showed antibacterial activity against 42 strains of Gram-positive cocci and 10 strains of Gram-positive bacilli,its inhibition zone diameters for Staphylococcus epidermidis,S.aureus,S.intermedius and Gram-positive Corynebacterium were not significantly different from those of levofloxacin(all at P>0.05).Its MIC range of diacetate against Staphylococcus epidermidis,S.aureus,S.intermedius and other Staphylococci was 1-32μg/ml,and its respective MIC90 was 16,8,16,and 32μg/ml.Diacerein had no bacteriostatic effect on 23 strains of Gram-negative bacilli,10 strains of filamentous fungi and 3 strains of candida.Conclusions Diacerein has antibacterial effects against Gram-positive Staphylococcus and Corynebacterium isolated from the ocular surface,but shows no antimicrobial activity against Gram-negative bacilli and fungi.Diacerein offers a new drug option and method for the treatment of bacterial keratitis.