蓝狐发情期及妊娠期血清五种生殖激素含量变化

Changes of Five Reproductive Hormones in Blue Fox during Estrus and Pregnancy

为探究发情期及妊娠期雌性蓝狐(Alopex lagopus)生殖激素的变化规律,选取11只繁殖期雌性蓝狐,采用放射免疫法(RIA)测定血清中雌二醇(E2)、促黄体生成素(LH)、促卵泡素(FSH)、孕酮(P)以及催乳素(PRL)的含量。结果表明,促卵泡素和促黄体生成素在发情期及妊娠期血清中浓度一直处于较稳定水平,并呈波动式分泌,最大值分别为(2.18±0.69)U/L和(8.82±1.83)U/L,同种激素不同检测日期数据两两比较差异不显著(P> 0.05);雌二醇在发情期和妊娠期血清中浓度亦处于较稳定水平,最大值出现在发情第1天,为(27.73±23.19)ng/L,数据两两比较差异亦不显著(P> 0.05);孕酮在发情期维持较高水平,最大值在发情第3天,为(2.41±1.35)μg/L,之后在妊娠期持续下降,分娩当天达到最低点,为(0.13±0.14)μg/L,数据两两比较差异显著(P <0.05);催乳素在发情期先下降,最低值为(0.15±0.04)U/L,第3天开始升高,并在妊娠期保持较高水平,到分娩时维持最高(0.21±0.05)U/L,数据两两比较差异不显著(P> 0.05)。初步探索了5种激素含量在发情和妊娠期间的动态变化,为养殖蓝狐的生殖生理提供参考数据。

In order to explore the changes in reproductive hormones in female blue foxes(Alopex lagopus) during estrus and gestation, 11 breeding individuals were selected and their serum levels of estradiol(E2), luteinizing hormone(LH), follicle-stimulating hormone(FSH), progesterone(P) and prolactin(PRL) were determined by radioimmunoassay(RIA). The results showed that the serum concentrations of follicle stimulating hormone and luteinizing hormone in estrus and gestation were at a relatively stable level and showed a fluctuating secretion pattern. The maximum values in the two periods were 2.18 ± 0.69 U/L and 8.82 ± 1.83 U/L, respectively. The serum concentration of estradiol in estrus and gestation was also at a relatively stable level, with the maximum value at 27.73 ± 23.19 ng/L on the fist day of estrus, but the overall values were not significantly different from each other(P > 0.05). Progesterone remained at a high level during estrus, with the maximum value synchronizing with estradiol 2.41 ± 1.35 μg/L on the third day, which continued to decline during gestation, and reached the lowest point on the day of delivery 0.13 ± 0.14 μg/L. The overall values were significantly different from each other(P < 0.05). Prolactin first decreased during estrus, the lowest value was 0.15 ± 0.04 U/L, and then increased on the 3 nd day. The level remained high during pregnancy, and reached the highest level 0.21 ± 0.05 U/L at the time of delivery, with no significant difference in the overall value(P > 0.05). The dynamic changes of each index during this period were preliminarily explored to provide reference data for reproductive physiology of blue fox.