有效抗病毒治疗后感染CXCR4 CCR5嗜性病毒的HIV感染者细胞内残留病毒的比较

A comparative study of intracellular residual viruses in different HIV tropism patients after effective antiviral therapy

目的探讨抗病毒治疗(ART)后病毒载量稳定在检测限以下的艾滋病病毒(HIV)感染者辅助受体使用与HIV库之间的关系。方法通过病毒env-脱氧核糖核酸(DNA)测序和geno2pheno预测HIV辅助受体的使用是CCR5还是CXCR4,用广州海力特生物科技有限公司试剂盒检测血液中的HIV DNA及未剪切的HIV核糖核酸(RNA)(usRNA)。结果共收集67例HIV感染者,CCR5嗜性感染者37例,CXCR4嗜性感染者30例。CXCR4嗜性的HIV DNA水平为(3.435±0.587)拷贝/106外周血单个核细胞(PBMC),CCR5嗜性为(2.938±0.664)拷贝/106 PBMC,两者差异有统计学意义(P<0.01);CXCR4嗜性的usRNA水平为(3.714±0.448)拷贝/106 PBMC,CCR5嗜性为(3.122±0.611)拷贝/10^6 PBMC,两者差异有统计学意义(P<0.001)。结论 CXCR4嗜性病毒和较高的HIV库相关。

Objective To investigate the relationship between the use co-receptor and HIV reservoir in patients with stable viral load below the detection line after ART. Methods A total of 67 patients were enrolled. Virus env-DNA sequencing and geno2 pheno were used to predict CCR5 or CXCR4 co-receptor used. HIV in 37 patients used CCR5 co-receptor and 30 patients used CXCR4 co-receptor. The HIVDNA and un-spliced HIV RNA(usRNA) in blood were detected with the kit of Guangzhou Hailite Biotechnology Co.,Ltd. Results In cases of CXCR4 patients, the level of HIVDNA was significantly higher than that in CCR5,with an average of(3.435±0.587)and(2.938±0.664)copies/10^6 respectively(P<0.01). The level of usRNA in CXCR4 patients was significantly higher than that in CCR5, with an average of(3.714±0.448) and(3.122±0.611) respectively(P<0.001). Conclusion CXCR4 tropic viruses are associated with higher HIV reservior.