QuEChERS-高效液相色谱法分析6-苄氨基腺嘌呤和吡唑醚菌酯在番茄植株和土壤中的残留动态

Residue kinetics analysis of 6-benzylaminopurine and pyraclostrobin in tomato plants and soils by QuEChERS-high performance liquid chromatography

为了探究根部处理剂6-苄氨基腺嘌呤(6-BA)和吡唑醚菌酯在植物-土壤间的分布规律,以番茄为试材,建立了测定番茄根、叶及根围土壤中2种化合物的QuEChERS-高效液相色谱法,并采用该方法研究了两者在番茄体内和根围土壤中28 d内的含量变化。结果表明:6-BA与吡唑醚菌酯在番茄根、叶和根围土壤中的回收率在82%~107%之间,定量限在0.03~0.09 mg/kg之间;经番茄苗蘸根处理后12 h^28 d,在根围土壤中未检测到6-BA,而在番茄叶和根中均有检出(0.043~2.0 mg/kg),表明6-BA可被根部快速吸收,并传导至叶片,在番茄各部位中的含量为侧叶>根部>顶叶;6-BA在番茄根部和叶片中消解较快,处理7~10 d后低于检出限;吡唑醚菌酯可扩散至根围土壤中,大部分被根部吸收并维持在较高含量水平(1.2~3.0mg/kg)达28 d以上;根部的吡唑醚菌酯可转移至叶片,并以较低含量水平(0.11~0.78 mg/kg)保留4~6 d。研究结果为番茄根、叶及土壤中6-BA和吡唑醚菌酯的残留分析提供了方法,并为评价该根部处理剂的应用效果提供了必要数据。

In order to investigate the distribution behavior of 6-benzylaminopurine(6-BA)and pyraclostrobin(two active ingredients for root-treatment agent)in plants,tomato was used as a model plant.A QuEChERS-high performance liquid chromatography method was developed to determine their dynamic contents in tomato roots,leaves and the rhizosphere soils.Results showed that the recoveries of 6-BA and pyraclostrobin in tomato and the rhizosphere soil were in the range of 82%-107%and the quantitative limit(LOQ)range was 0.03-0.09 mg/kg.After the root treatment,there was no 6-BA detected in the soil,but it was detected in the tomato roots and leaves in the range from 0.043 to 2.0 mg/kg,indicating the bio-availability by plants was very high.6-BA can be quickly absorbed by the roots and transferred to the side-leaf and the top-leaf.The order of distribution levels is side-leaf>root>top-leaf.6-BA degraded at a high speed in both tomato roots and the leaves.Thus,it was not detected 7-10 days after the treatment.A proportion of pyraclostrobin was distributed into the soil and most of that proportion was absorbed by roots with a high level(1.2-3.0 mg/kg)for 28 days.A small amount of pyraclostrobin in roots was transferred to leaves and was kept with a level of 0.11-0.78 mg/kg for 4-6 days.This study has developed a method for the residue analysis of 6-BA and pyraclostrobin in the tomato root,leaf and soil,and it has also provided necessary data for evaluating the application of the root-treatment agent.