小胶质细胞培养上清液对鼻咽癌细胞迁移和侵袭能力的影响

Effect of Microglia Cell Supernatant on Migration and Invasion of Nasopharyngeal Carcinoma Cells

目的:研究未活化和活化态人小胶质细胞系CHME-5培养上清液对鼻咽癌细胞CNE-2迁移、侵袭能力的影响。方法:采用1μg/mL LPS活化人小胶质细胞系CHME-5,收集未活化和LPS活化的CHME-5培养上清液。分为对照组(无血清培养基)、45%上清组、90%上清组、LPS组(LPS干预的小胶质细胞上清)、45%LPS上清组、90%LPS上清组。使用划痕实验和Transwell小室建立鼻咽癌细胞CNE-2的迁移模型,观察不同浓度的未活化/活化小胶质细胞培养上清液对鼻咽癌细胞迁移能力的影响;使用Transwell小室建立鼻咽癌细胞CNE-2的侵袭模型,用结晶紫染色观察不同浓度的未活化/活化小胶质细胞培养上清液对鼻咽癌细胞侵袭能力的影响。结果:低浓度(45%)和高浓度(90%)未活化/活化的小胶质细胞培养上清液均促进鼻咽癌细胞迁移和侵袭。结论:无论小胶质细胞是否活化,其培养上清液均促进鼻咽癌细胞的迁移和侵袭。

Objective: To study the effect of unactivated/activated human microglia cell line CHME-5 on the migration and invasion of nasopharyngeal carcinoma cell line CNE-2. Methods: Human microglia cell line CHME-5 was cultured, and 1 μg/mL LPS was used to activate cells. Unactivated/activated cell supernatant was collected. For the experiment, we established 6 groups: control group, 45% supernatant group, 90% supernatant group, LPS group(LPS activated microglia supernatant), 45% LPS supernatant group, and 90% LPS supernatant group. The wound healing assay and Transwell inserts were used to establish the nasopharyngeal carcinoma cell line CNE-2 migration model, and the effect of unactivated/activated microglia supernatant on the migration of the cell was observed. Transwell inserts were used to establish the nasopharyngeal carcinoma cell line CNE-2 invasion model, and crystal violet staining was used to observe the effect of microglia supernatant on the migration of the cell. Results: Low(45%) and high(90%) concentrations of unactivated/activated microglia supernatant promoted the migration and invasion of nasopharyngeal cancer cells. Conclusion: Both unactivated and activated microglia supernatant promote the migration and invasion of nasopharyngeal carcinoma cells.