KIFC3基因在结直肠癌中的表达及预后分析

Analysis of KIFC3 Gene Expression in Colorectal Cancer and Its Prognosis

目的比较驱动蛋白家族分子C3(KIFC3)基因在结直肠癌组织中的表达及对预后的影响。方法从肿瘤基因组图谱(TCGA)和Oncomine数据库中收集结直肠癌RNA表达数据,分析KIFC3的表达情况;收集2017年1-6月汕头大学医学院附属第一医院收治的19例结直肠癌切除标本,采用实时荧光定量聚合酶链反应检测癌组织及癌旁正常组织中KIFC3的表达。结合TCGA结直肠癌患者的临床资料,比较各项临床病理特征中KIFC3的表达水平;采用Kaplan-Meier plotter和Cox回归比较KIFC3高表达组与低表达组患者的预后。结果 KIFC3在结直肠癌中的表达显著高于正常组织(3. 39±6. 26比2. 07±4. 93)(P <0. 05)。结直肠癌患者中发生淋巴结转移的KIFC3 mRNA水平高于无转移者(8. 42±0. 97比8. 05±1. 05),肿瘤浸润T3~4期的KIFC3 mRNA水平高于肿瘤浸润T1~2期的患者(8. 30±1. 01比7. 87±1. 03),病理分期Ⅲ~Ⅳ期的KIFC3 mRNA水平高于Ⅰ~Ⅱ期患者(8. 38±0. 95比8. 06±1. 07)(P <0. 01)。KIFC3表达水平越高,结直肠癌患者总生存时间越短(P <0. 05),KIFC3高表达患者的死亡风险约为低表达患者的1. 8倍(HR=1. 808,95%CI 1. 012~3. 229,P=0. 045)。结论 KIFC3在结直肠癌组织中表达升高,KIFC3高表达的结直肠癌患者预后更差。

Objective To investigate the expression of kinesin family member C3( KIFC3) in colorectal cancer and its effect on the prognosis. Methods RNA expression datasets of colorectal cancer were downloaded from The Cancer Genome Atlas( TCGA) and Oncomine databases,and mRNA expression of KIFC3 was analyzed. The specimens were collected from19 colorectal cancer patients treated in the First Affiliated Hospital of Shantou University Medical College from January to June 2017. The mRNA expression of KIFC3 in cancer and adjacent tissues was detected by real-time quantitative polymerase chain reaction. The relationship between KIFC3 expression and clinicopathological features of colorectal cancer patients from TCGA database was analyzed. Kaplan-Meier plotter and Cox regression were used to analyze the correlation between KIFC3 expression and the prognosis. Results The mRNA expression of KIFC3 in colorectal cancer tissues was significantly higher than that in normal tissues( 3. 39 ± 6. 26 vs 2. 07 ± 4. 93)( P < 0. 05). The KIFC3 mRNA level of lthe patients with metastasis was higher than that in the patients without metastasis( 8. 42 ± 0. 97 vs 8. 05 ± 1. 05);the KIFC3 mRNA level of the patients with tumor invasion T3-4 was higher than that in the patients with tumor invasion T1-2( 8. 30 ± 1. 01 vs 7. 87 ±1. 03);the level of KIFC3 mRNA in pathological stage Ⅲ-Ⅳ was higher than that in stage Ⅰ-Ⅱ( 8. 38 ± 0. 95 vs 8. 06 ±1. 07)( P < 0. 01). The overall survival time of the patients with high expression of KIFC3 was shorter than that of the patients with low expression of KIFC3( P < 0. 05),and the risk of death in the patients with high expression of KIFC3 was approximately 1. 8 times of that of the patients with low expression( HR = 1. 808,95% CI 1. 012-3. 229,P = 0. 045).Conclusion KIFC3 is over-expressed in colorectal cancer tissues;and the patients with high expression of KIFC3 have worse prognosis.