单碱基编辑技术的研究进展

Research Progress of Single Base Editing Technology

早期点突变校正方法的第一步切割DNA双链通常会引入大量随机插入和缺失,效率低下,增加基因编辑的风险。单碱基编辑器以可编程的方式将目标碱基进行直接、不可逆的转换,无需DNA双链的断裂或外源模板的引入,展示出在基因治疗、生物定向改造等方面的巨大应用潜力。

Cutting DNA double strands,the first step of the early point mutation correction method,usually introduces a large number of random insertions and deletions,which is inefficient and increases the risk of gene editing.The single base editor can convert the target base directly and irreversibly in a programmable way without the breaking DNA double chains or introducing exogenous templates,thus showing great application potential in gene therapy,biological targeted modification and so on.