脂联素基因rs2241766 多态性与代谢综合征的相关性研究

Association Study between the Polymorphism of Adiponectin Gene rs2241766 and Metabolic Syndrome

目的探讨脂联素(adiponectin,APN)rs2241766基因多态性与代谢综合征(metabolic syndrome,MS)患者发生的关系。方法410例MS患者和203例健康人群纳入研究。使用酶联免疫吸附试验(ELISA)检测研究对象的血清APN水平,采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术检测APN基因rs2241766位点T/G基因的多态性,分析各基因型与MS发生的关系。结果MS患者血清APN水平明显低于对照组(15.3±4.4 mg/ml vs 27.2±6.5 mg/ml),差异有统计学意义(χ^2=41.7,P<0.001);rs2241766位点三种基因型在MS组与对照组间的分布,差异有统计学意义(χ^2=39.222,P<0.001),MS组G等位基因频率高于健康人群,差异有统计学意义(χ^2=36.657,P<0.001);logistic回归分析显示研究组G等位基因发生MS的风险高于T等位基因(OR=2.19,95%CI:1.17~3.01 vs OR=1.00ref),差异有统计学意义(P=0.034,<0.05)。TG和GG基因型发生MS的风险较TT基因型增高(OR=1.55,95%CI:0.79~2.83;OR=2.48,95%CI:1.67~7.35 vs OR=1.00ref),将TG和GG基因型合并后发生MS风险同样增高(OR=2.23,95%CI:1.21~6.09),差异均有统计学意义(P=0.019,0.006,0.029),且TG(15.7±4.1 mg/ml)及GG(14.3±4.5 mg/ml)基因型研究对象的APN水平低于TT基因型(18.7±4.9 mg/ml),差异有统计学意义(F=7.621,P<0.001)。结论MS的发生可能与rs2241766位点的基因变异相关,尤其是T向G变异的个体发生MS的风险更高,使APN水平降低。

Objective To investigate the association between adiponectin( APN) rs2241766 gene polymorphism and patients with metabolic syndrome( MS). Methods 410 patients with MS and 203 healthy subjects were included in the study. The serum APN level of the subjectswas detected by enzyme-linked immunosorbent assay( ELISA),and the polymorphism of APN gene locus rs2241766 T/G was detected by polymerase chain reaction-restriction fragmentlength polymorphism( PCR-RFLP),analyzing the relationship that occurs with MS. Results Serum APN levels in the MS group were significantly lower than those in the control group( 15. 3 ± 4. 4 mg/ml vs 27. 2 ± 6. 5 mg/ml),the difference was statistically significant( t = 41. 7,P < 0. 001). The distribution of the three genotypes of rs2241766 between MS patients and the control group were statistically significant( χ~2= 39. 222,P < 0. 001),and the G allele frequency of MS patients was higher than healthy population,the difference was statistically significant( χ~2= 36. 657,P < 0. 001). Logistic regression analysis showed that the risk of MS in the G allele was higher than that in the T allele( OR = 2. 19,95% CI: 1. 17 ~ 3. 01 vs OR = 1. 00 ref),the difference was statistically significant( P = 0. 034),and the risk of MS in TG and GG genotypes was higher than that in the TT genotype( OR= 1. 55,95% CI: 0. 79 ~ 2. 83;OR = 2. 48,95% CI: 1. 67 ~ 7. 35 vs OR = 1. 00 ref),the risk of MS was still increased after the combination of TG and GG genotypes( OR = 2. 23,95% CI: 1. 21 ~ 6. 09),the difference was statistically significant( P = 0. 019,0. 006,0. 029). And the APN levels of the TG( 15. 7 ± 4. 1 mg/ml) and GG( 14. 3 ± 4. 5 mg/ml) subjects were lower than those of the TT( 18. 7 ± 4. 9 mg/ml),the difference was statistically significant( F = 7. 621,P < 0. 001).Conclusion The occurrence of MS may be related to the site variation of rs2241766,especially the individuals with T-to-G variation have higher risk of MS and lower APN level.