精浆8-OHdG和GSTs水平与男性不育的相关性研究

Study on the Correlation between 8-OHdG and GSTs Levels in Seminal Plasma and Male Infertility

目的探讨精浆8-羟基脱氧鸟苷(8-hydroxydeoxyguanosine,8-OHdG)和谷胱甘肽硫转移酶(glutathioneS-transferases,GSTs)水平与男性不育疾病的相关性。方法收集2017年5月~2019年5月期间在陕西省咸阳市第一人民医院和渭南市蒲城县医院就诊的35例不育男性患者的精液标本进行分析。同时收集婚后已生育的26例健康男性的精液标本作为对照组。分析两组精液质量、精浆8-OHdG和GSTs水平、血清睾酮(testosterone,T),卵泡刺激素(follicle stimulating hormone,FSH)及黄体生成素(luteinizing hormone,LH)水平的变化及其相关性。精浆8-OHdG和GSTs水平采用酶联免疫吸附法测定。精液质量采用伟力CASA 900系统进行分析。结果对照组的精浆8-OHdG(pg/ml)和GSTs(U/ml)水平分别为87.13±26.30和25.34±3.09,不育组的精浆8-OHdG(pg/ml)和GSTs(U/ml)水平分别为156.37±52.31和12.71±1.25。与对照组比较,不育组精浆8-OHdG水平显著增高,而GSTs水平则显著降低,差异均有统计学意义(t=26.43~45.21,P=0.000)。对照组的精液总量(ml)、精子密度(×10^6/ml)、精子活力(%)和精子畸形率(%)分别为3.16±0.28,83.23±22.28,62.27±5.13和7.23±2.39。不育组的精液总量(ml)、精子密度(×10^6/ml),精子活力(%)和精子畸形率(%)分别为3.02±0.25,5.32±3.90,10.23±2.18和49.56±7.29。与对照组比较,不育组的精子密度及精子活力显著降低,而精子畸形率则显著增高,差异有统计学意义(t=43.64~69.26,P=0.000)。不育组和对照组的精液总量比较,差异无统计学意义(t=0.399,P=0.732)。对照组的血清T(nmol/ml),FSH(mIu/ml)及LH(mIu/ml)水平分别为19.23±5.38,5.83±1.37,5.19±2.24。不育组的血清T(nmol/ml),FSH(mIu/ml)及LH(mIu/ml)水平分别为7.97±3.62,19.90±3.36和15.53±5.13。与对照组比较,不育组的FSH及LH水平显著增高,T水平则显著降低,差异均有统计学意义(t=17.92~31.23,P=0.000)。精浆8-OHdG水平与精子密度、精子活力和血清T水平有负相关性,而与精子畸形率、血清FSH及LH水平有正相关性(r=-0.823,-0.819,-0.798,0.767,0.782,0.807,均P<0.01)。精浆GSTs水平与精子密度、精子活力和T水平有正相关性,而与精子畸形率、血清FSH及LH水平有负相关性(r=0.857,0.842,0.819,-0.838,-0.802,-0.814,均P<0.01).结论8-OHdG和GSTs通过调节机体的氧化应激能力影响精子质量,检测精浆8-OHdG和GSTs水平可以为男性不育疾病的诊治提供依据。

Objective To investigate the correlation between the levels of 8-hydroxydeoxyguanosine(8-OHdG)and glutathione S-transferases(GSTs)in seminal plasma and male infertility.Methods The semen samples of 35 infertile males were collected to analyze from May 2017 to May 2019 in the First People’s Hospital of Xianyang and Pucheng County Hospital of Weinan City.At the same time,the semen samples of 26 healthy male were collected as control group.The changes and correlation of semen quality,levels of 8-OHdG and GSTs in seminal plasma,levels of testosterone(T),follicle stimulating hormone(FSH)and luteinizing hormone(LH)in serum between the two groups were analyzed.The levels of 8-OHdG and GSTs in seminal plasma were determined by ELISA.Semen was analyzed by Weili CASA 900 system.Results The seminal plasma levels of 8-OHdG(pg/ml)and GSTs(U/ml)in the control group were 87.13±26.30 and 25.34±3.09,while those of 8-OHd G(pg/ml)and GSTs(U/ml)in the infertile group were 156.37±52.31 and 12.71±1.25,respectively.The seminal plasma levels of 8-OHdG were significantly higher whereas of GSTs were significantly lower in the infertile group compared with those in the control group the difference was statistically significant(t=26.43~45.21,P=0.000).The total semen(ml),sperm density(×106/ml),sperm motility(%)and sperm malformation rate(%)in the control group were 3.16±0.28,83.23±22.28,62.27±5.13 and 7.23±2.39,respectively.The total semen(ml),sperm density(×106/ml),sperm motility(%)and sperm malformation rate(%)in the infertile group were 3.02±0.25,5.32±3.90,10.23±2.18 and 49.56±7.29,respectively.The sperm density and motility in infertile group were significantly lower than those in control group,while the rate of sperm malformation was significantly higher than that in control group,the difference was statistically significant(t=43.64~69.26,P=0.000).There was no statistically statisticant difference in semen volume between the two groups(t=0.399,P=0.732).The levels of serum T(nmol/ml),FSH(mIu/ml)and LH(mIu/ml)in the control group were 19.23±5.38,5.83±1.37 and 5.19±2.24,respectively.The levels of serum T(nmol/ml),FSH(m Iu/ml)and LH(m Iu/ml)in the infertile group were 3.02±0.25,5.32±3.90,10.23±2.18 and 49.56±7.29,respectively.Compared with the control group,the levels of FSH and LH in the infertility group increased significantly,while the levels of T decreased significantly(t=17.92~31.23,P=0.000).The levels of 8-OHdG in seminal plasma were negatively correlated with sperm density,sperm motility and serum T level,and positively correlated with sperm malformation rate,serum FSH and LH levels(r=-0.823,-0.819,-0.798,0.767,0.782,0.807,all P<0.01).The level of GSTs in seminal plasma were positively correlated with sperm density,sperm motilityand T level,but negatively correlated with sperm malformation rate,FSH levels and LH levels(r=0.857,0.842,0.819,-0.838,-0.802,-0.814,all P<0.01).Conclusion 8-OHdG and GSTs could affect sperm quality by regulating oxidative stress,and detection of the levels of 8-OHdG and GSTs in seminal plasma can provide evidence for the diagnosis and treatment of male infertility.