宫颈鳞癌及正常宫颈组织细胞中TAZ的表达分析

Expression of TAZ in cervical squamous cell carcinoma and normal cervical tissue cells

目的:分析转录共激活因子PDZ结合基序(transcriptional co-activator with PDZ-binding motif,TAZ)蛋白在宫颈鳞癌组织细胞中的表达意义。方法:免疫组化、免疫荧光及western-blot检测正常宫颈组织、宫颈鳞癌组织中TAZ蛋白的表达及亚细胞定位,分析其与宫颈鳞癌临床病理特征之间的关系。结果:TAZ在正常宫颈组织细胞表达阳性率为100%,宫颈鳞癌组织细胞为85.85%,差别有统计学意义(P<0.05);宫颈癌不同分化程度、有淋巴结转移组与无淋巴结转移组比较,TAZ的表达差异均有统计学意义(P<0.05)。结论:TAZ蛋白在宫颈鳞癌中的表达与肿瘤分化程度及有无淋巴结转移有关。

Objective:To investigate the expression of TAZ protein in cervical squamous cell carcinoma(CSCC)tissue cells.Methods:Immunohistochemistry,immunofluorescence assay,and Western blot were used to measure the expression of TAZ protein in normal cervical tissue and CSCC tissue and determine its subcellular localization,and the association between the expression of TAZ protein and the clinicopathological features of CSCC was analyzed.Results:There was a significant difference in the positive rate of TAZ between normal cervical tissue cells and CSCC tissue cells(100%vs 85.85%,P<0.05).There was a significant difference in the expression of TAZ between the groups with different degrees of tumor differentiation and between the group with lymph node metastasis and the group without lymph node metastasis(P<0.05).Conclusion:The expression of TAZ protein in CSCC is associated with the degree of tumor differentiation and the presence or absence of lymph node metastasis.