lncRNA ABHD11-AS1在宫颈癌中的表达及其临床意义

Expression and clinical significance of long non-coding RNA ABHD11-AS1 in cervical cancer

目的:探讨长链非编码lncRNA ABHD11-AS1在宫颈癌中的表达及其与患者诊断及预后的关系。方法:采用实时荧光定量PCR检测方法,检测50例宫颈癌患者的血液标本及对照组中lncRNA ABHD11-AS1的表达水平,同时分析lncRNA ABHD11-AS1的相对表达量与宫颈癌患者诊断、临床病理特征及预后的关系。结果:lncRNA ABHD11-AS1在宫颈癌患者血液标本及对照组中的相对表达水平分别为(1.80±0.11)和(1.17±0.08),差异有统计学意义(P<0.05)。lncRNA ABHD11-AS1的表达水平与宫颈癌患者的淋巴结转移,淋巴管血管侵袭、肿瘤大小及FIGO分期有关(P<0.05),而与患者的年龄、绝经情况等均无关,同时ROC显示lncRNA ABHD11-AS1作为宫颈癌标志物具有一定的诊断意义。与lncRNA ABHD11-AS1低表达组比较,lncRNA ABHD11-AS1高表达组患者的中位OS明显缩短(P<0.05)。结论:lncRNA ABHD11-AS1在宫颈癌中的表达水平升高,lncRNA ABHD11-AS1可以作为判断宫颈Ca预后的分子标志物。

Objective:To investigate the expression of the long non-coding RNA(lncRNA)ABHD11-AS1 in cervical cancer and its association with diagnosis and prognosis.Methods:Quantitative real-time PCR was used to measure the expression level of lncRNA ABHD11-AS1 in blood samples of 50 patients with cervical cancer and the subjects in the control group,and the association of the relative expression level of lncRNA ABHD11-AS1 with the diagnosis,clinicopathological features,and prognosis of cervical cancer patients was analyzed.Results:There was a significant difference in the relative expression level of lncRNA ABHD11-AS1 in blood samples between the patients with cervical cancer and the control group(1.80±0.11 vs 1.17±0.08,P<0.05).The expression level of lncRNA ABHD11-AS1 was associated with lymph node metastasis,lymphatic vessel invasion,tumor size,and FIGO stage in patients with cervical cancer(P<0.05),while it was not associated with patients’age and menopause status.The receiver operating characteristic curve analysis showed that lncRNA ABHD11-AS1 had certain diagnostic significance as a marker for cervical cancer.Compared with the low lncRNA ABHD11-AS1 expression group,the high lncRNA ABHD11-AS1 expression group had a significantly shorter median OS(P<0.05).Conclusion:The expression of lncRNA ABHD11-AS1 is upregulated in cervical cancer,and lncRNA ABHD11-AS1 can be used as a potential molecular marker for diagnosis and prognosis.