阿奇霉素对阿霉素与白蛋白致小鼠肾损害的保护作用

Protective effects of azithromycin on adriamycin-induced nephropathy with albumin overload in mice

目的:研究阿奇霉素对阿霉素与白蛋白致小鼠肾损害的肾脏保护作用。方法:取BALB/c雄性小鼠40只,按照随机数表法平均分为空白对照组(Ctrl组)、肾损害模型组(ADR+BSA组)、阿奇霉素治疗组(Azm组)及醋酸泼尼松阳性对照组(Pdn组);ADR+BSA、AZM及Pdn三组每周5 d尾静脉注射9.8 mg·kg-1阿霉素,腹腔注射10 mg·kg-1血清白蛋白,对照组注射生理盐水,持续4周造模;之后,AZM组每天给予62.5 mg·kg-1阿奇霉素灌胃,Pdn组每天给予12.5 mg·kg-1醋酸泼尼松灌胃,其余两组给予等量生理盐水,持续6周后,收集并记录24 h尿量,检测尿蛋白量、内生肌酐清除率,取血检测血清生化指标和免疫因子。结果:与Ctrl组相比,ADR+BSA组小鼠24 h尿蛋白定量显著升高(P<0.05),Ccr显著降低(P<0.05);经过阿奇霉素治疗后的小鼠,24 h蛋白定量相比于ADR+BSA组显著降低(P<0.05),Ccr显著升高(P<0.05)。结论:阿奇霉素对阿霉素与白蛋白致小鼠的肾损害有一定的保护作用。

Objective:To study the protective effects of azithromycin on renal damage induced by doxorubicin and albumin in mice.Methods:Forty male BALB/c mice were randomly divided into blank control group(Ctrl group),renal damage model group(ADR+BSA group),azithromycin treated group(Azm group)and prednisone acetate positive control group(Pdn group)in accordance with random number table method.Mice in ADR+BSA,AZM and Pdn group were injected intravenously with 9.8 mg·kg-1 doxorubicin five days a week,10 mg·kg-1 serum albumin was injected intraperitoneally,and normal saline was administered to the control group for 4 weeks to establish renal damage model.After that,AZM group was given daily.62.5 mg·kg-1 azithromycin was intragastrically administered.The Pdn group was given 12.5 mg·kg-1 prednisone acetate daily,the other two groups were given the same amount of normal saline.After 6 weeks,the urine volume was collected and recorded for 24 hours to detected urine protein amount and endogenous creatinine clearance rate(Ccr).Serum biochemical indicators and serum immune factors were detected.Results:Compared with the Ctrl group,the 24 h urine protein level of the ADR+BSA group was increased significantly(P<0.05),and the Ccr was decreased significantly(P<0.05).After the azithromycin treatment,the 24 h urine protein was decreased significantly(P<0.05),while the Ccr was increased significantly(P<0.05)compared with ADR+BSA group.Conclusion:Azithromycin has a protective effects on the renal damage induced by doxorubicin and albumin in mice.