益生菌对高尿酸血症肾功能损伤的疗效分析研究

Analysis of Therapeutic Effects of Probiotics on Renal Injury Induced by Hyperuricemia

背景别嘌醇被认为是临床治疗高尿酸血症的有效药物,但是别嘌醇对于高尿酸血症肾功能损伤时期治疗仍存在争议,多数学者认为别嘌醇可以有效保护肾脏,但是由于可能会出现肾功能损伤而被限制使用。目前,益生菌已被证明可以有效降解尿酸(UA)。目的从传统泡菜中筛选出益生菌,分析其对高尿酸血症肾功能损伤的疗效。方法于2017年12月-2018年8月采集中国不同省份地区的18种传统泡菜,从不同种类的泡菜中分离出乳酸菌,首先通过双氧水(H2O2)、HE染色后镜检进行初步确定,随后筛选出具有降核苷,对酸性、胆盐、胰蛋白酶、胃蛋白酶耐受性、黏附性良好的功能性乳酸菌,用16SrDNA序列分析对菌种进行鉴定,并最终应用于高尿酸血症肾功能损伤模型。采用随机数字表法将40只大鼠分为正常组、模型组、S12菌株干预组、别嘌醇干预组,各10只。在第0、10、20天测定UA、血肌酐(Scr)、尿素氮(BUN)水平。结果 18种泡菜中分离出14株乳酸菌,其中S1、S5、S12、S14作为候选菌株。因S12菌株提纯度最高,因此以此进行动物阶段实验。第10天时,四组Scr水平比较,差异无统计学意义(P>0.05);第10天时,四组UA、BUN水平比较,差异均有统计学意义(P<0.05);其中模型组、S12菌株干预组、别嘌醇干预组UA、BUN水平均高于正常组(P<0.05)。第20天时,四组UA、Scr、BUN水平比较,差异均有统计学意义(P<0.05);其中模型组、S12菌株干预组、别嘌醇干预组UA、Scr、BUN水平均高于正常组,S12菌株干预组UA、Scr、BUN水平均低于模型组,别嘌醇干预组UA水平低于模型组和S12菌株干预组,BUN水平低于模型组、高于S12菌株干预组(P<0.05)。结论益生菌对高尿酸血症肾功能损伤时期具有一定程度的保护治疗作用。

Background The treatment of hyperuricemia with allopurinol is considered to be clinically effective.However,the treatment of renal injury caused by hyperuricemia with allopurinol is still controversial.Most scholars believe that allopurinol can effectively protect the kidney,but its use was limited due to the possible side effects of renal injury.Currently,probiotics have been shown to effectively degrade uric acid(UA).Objective Probiotics were screened from traditional pickles to analyze their effects on hyperuricemia with renal injury.Methods From December 2017 to August 2018,lactic acid bacteria(LAB) were isolated from 18 kinds of pickles collected from different provinces.Firstly,functional LAB with good adhesion,which could degrade nucleosides and were resistant to acid,bile salts,trypsin and pepsin,were selected by microscopic examination after hydrogen peroxide(H2 O2) and HE staining.The strains were identified by 16 SrDNA sequence analysis and finally applied to the renal injury model of hyperuricemia.A total of 40 rats were divided into normal group(n=10),model group(n=10),S12 strain intervention group(n=10) and allopurinol intervention group(n=10) by random number table method.Levels of UA,serum creatinine(Scr) and urea nitrogen(BUN) were measured on days 0,10 and 20.Results Fourteen strains of LAB were isolated from 18 kinds of pickles,among which S1,S5,S12 and S14 were used as candidate strains.The purity of S12 strain was the highest,so it was used for animal test.At day 10,Scr level in four groups showed no significantly difference(P>0.05),while the levels of UA and BUN in four groups were significantly different(P<0.05).UA and BUN levels in the model group,S12 strain intervention group and allopurinol intervention group were higher than those in the normal group(P<0.05).On the day 20,there were significant differences in the levels of UA,Scr and BUN in four groups(P<0.05);among them,UA,Scr and BUN levels in the model group,S12 strain intervention group,and allopurinol intervention group were higher than those in the normal group;UA,Scr and BUN levels in the S12 strain intervention group were lower than those in the model group;UA and BUN levels in allopurinol intervention group were lower than those in the model group,while BUN level in allopurinol intervention group was higher than that in the S12 strain intervention group(P<0.05).Conclusion Probiotics have a certain degree of protective and therapeutic effects on hyperuricemia with renal injury.