2018-2019年流感流行季节河北省流感病毒对神经氨酸酶抑制剂药物的耐药性研究

Drug resistance analysis of influenza virus to neuraminidase inhibitor from 2018 to 2019 in Hebei Province

目的分析河北省2018-2019年流感流行季节流感病毒对神经氨酸酶抑制剂类(neuraminidase inhibitor,NAI)药物的耐药情况,为流感的预防控制提供依据。方法选取H1N1(pdm09)亚型毒株25株、H3N2亚型毒株11株共36株流感病毒毒株进行生物学耐药实验,使用荧光发光法检测病毒对奥司他韦和扎纳米韦的药物敏感性。结果 2018-2019年河北省H1N1(pdm09)和H3N2亚型流感病毒全部对奥司他韦和扎那米韦敏感,对奥司他韦的半数抑制浓度(half maximal inhibitory concentration,IC_(50))平均数分别为0.50 nM(0.07~1.14 nM)和0.25 nM(0.09~0.69 nM),对扎那米韦的IC_(50)平均数分别为0.29 nM(0.09~0.85 nM)和0.87 nM(0.17~1.81 nM),均在相对应型别参考株IC_(50)值10倍以内。结论 2018-2019年河北省检测的流感毒株都对NAI类药物敏感。

Objective To analyze the resistance of influenza virus to neuraminidase inhibitors(NAI)in Hebei province during 2018-2019.Methods Virus were collected from the Hebei Influenza Surveillance Network during 2018-2019.A total of 36 confirmed influenza viruses(with 25 H1 pdm09 and 11 H3 N2)were selected to test resistance to oseltamivir and zanamivi with fluorescence(FL).Results All 36 influenza viruses tested were sensitive to oseltamivir and zanamivir.The median half maximal inhibitory concentration(IC50)for oseltamivir of H1 pdm09 and H3 N2 were of 0.50 nM(range 0.07-1.14 nM)and 0.25 nM(range 0.09-0.69 nM)respectively,while 0.29 nM(range 0.09-0.85 nM)and 0.87(range 0.17-1.81 nM)for zanamivir,all were within 10 fold IC50 of the reference virus(corresponding type).Conclusion All the tested influenza strains isolated in Hebei province during 2018-2019 were sensitive to NAI.