重组人内抑素对大鼠骨关节炎关节软骨退变和自由基的影响

Effects of recombinant human endostatin on degeneration and free radical of articular cartilage in rat osteoarthritis

目的:探讨重组人内抑素(endostatin ES)对实验性大鼠骨关节炎(OA)软骨退变与自由基的影响,以探讨ES对大鼠骨关节炎的治疗作用。方法:Wistar大鼠关节腔注射4%木瓜蛋白酶建立OA模型,40只Wistar雄性大鼠随机分为4组,即正常组A、模型组B、ES 1.25mg/kg·d治疗组C、ES 2.5mg/kg·d治疗组D,末次造模一周后治疗组开始给药,治疗组皮下注射ES,连续7d,6周后处死动物。切除膝关节进行组织学检查,并检查关节液中超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量。结果:与模型组对比,ES 1.25mg/kg·d、2.5mg/kg·d可以提高关节液中SOD的活性,降低关节液中MDA的水平。可以明显改变OA大鼠膝关节病变。结论:ES可以有效的防治实验大鼠OA,其作用机制可能是通过提高关节液中SOD的活性,降低关节液中MDA的含量起作用。

Objective:To investigate the effects of recombinant human endostatin(ES)on the degeneration and free radical of articular cartilage in experimental rat osteoarthritis.Methods:Forty male Wistar rats as the research object,and randomly divided into 4 groups,the normal,Agroup,model group B,ES 1.25 mg/kg·d the treatment group C and ES 2.5 mg/kg·d,group D d treatment,other groups except normal group A rat articular cavity injection of papain 4%early osteoarthritis model is set up,the building after A week at the end of the treatment group began to medicine,the treatment group hypodermic ES,for seven consecutive days,six weeks after death of animals.The knee was removed for histological examination,and the activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)in the joint fluid were examined.Results:Compared with the model group,the treatment of ES could increase the activity of SOD and decrease the level of MDA in joint fluid.ES treatment can significantly change knee joint lesions of osteoarthritis rats.Conclusion:ES can effectively prevent and treat osteoarthritis in experimental rats,and the mechanism may be to improve the activity of SOD in joint fluid and reduce the content of MDA in joint fluid to play a protective role.