铁皮石斛多糖对脑缺血-再灌损伤模型大鼠预后的影响

Effect of dendrobium candidum polysaccharide on the prognosis of rat model of cerebral ischemia and reperfusion injury

目的:探索铁皮石斛多糖对脑缺血-再灌注(MCAO)模型大鼠预后的影响。方法:将100只雄性SD大鼠随机分为假手术组、模型组、不同浓度铁皮石斛多糖(100、200、300 mg/kg)组。建模后第一天起各铁皮石斛多糖组大鼠连续3d,每天一次腹腔注射铁皮石斛多糖溶液;在建模后第7天对比各组大鼠的存活率、脑梗死体积、改良神经功能评分(mNSS)以及血清炎症因子(TNF-α、IL-1β)的水平。结果:与模型组相比,各浓度铁皮石斛多糖组大鼠存活率均升高,其中300mg/kg铁皮石斛多糖组较模型组显著升高(P<0.05);与模型组相比,各浓度铁皮石斛多糖组脑梗死体积、mNSS、炎症因子(TNF-α、IL-1β)水平等指标均降低,其中200、300 mg/kg铁皮石斛多糖组各指标均较模型组显著降低(P<0.05)。结论:铁皮石斛多糖对脑缺血-再灌注损伤大鼠的预后具有促进作用,机制可能与降低模型鼠体内炎症因子的表达有关。

Objective:To investigate the effect of dendrobium candidum polysaccharide on the prognosis of cerebral ischemia-reperfusion(MCAO)rats.Methods:One hundred male SD rats were randomly divided into sham operation group,model group and dendrobium candidum polysaccharide(100,200 and 300 mg/kg)group.The rats in each dendrobium candidum polysaccharide group were intraperitoneally injected with dendrobium candidum polysaccharide solution once a day for 3 consecutive days from the first day after modeling.Survival,infarct volume,improved neurological function score(mNSS)and serum inflammatory cytokines(TNF-α,IL-1β)were compared on day 7 after modeling.Results:Compared with the model group,the survival rate of the polysaccharide group of dendrobium candidum increased,among which the polysaccharide group of 300 mg/kg was significantly higher than that of the model group(P<0.05).Compared with the model group,the indexes of cerebral infarction volume,mNSS,inflammatory factors(TNF-α,IL-1β)in the polysaccharide group of dendrobium candidum at various concentrations were all decreased,and the indexes of polysaccharide group of 200 and 300 mg/kg were significantly decreased compared with the model group(P<0.05).Conclusion:dendrobium candidum polysaccharides can promote the prognosis of rats with cerebral ischemia-reperfusion injury,and the mechanism may be related to the decreased expression of inflammatory factors in rats.