胃癌组织中miR-133a-3p、miR-4317的表达及临床意义

The expression and clinical significance of miR-133a-3p and miR-4317 in gastric cancer

目的检测胃癌组织中微小RNA(miR)-133a-3p和miR-4317表达情况,并探究二者的临床意义。方法收集60例胃癌患者的癌组织及其相对应的癌旁组织,采用实时荧光定量反转录PCR(RT-qPCR)技术检测组织样品中miR-133a-3p和miR-4317表达水平,同时分析miR-133a-3p和miR-4317表达与患者临床特征之间的关系,采用Kaplan-Meier生存曲线分析miR-133a-3p和miR-4317表达与患者预后的关系。结果RTqPCR试验结果显示,胃癌组织中miR-133a-3p、miR-4317相对表达量均低于癌旁正常组织,差异有统计学意义(P<0.05)。胃癌组织中miR-133a-3p表达水平与肿瘤TNM分期、淋巴结转移有关(P<0.05);miR-4317表达水平与淋巴结转移、远处转移及肿瘤TNM分期有关(P<0.05)。Kaplan-Meier生存曲线显示,miR-133a-3p高表达胃癌患者的5年生存率高于低表达者,术后生存时间长于低表达者(P<0.05);miR-4317低表达胃癌患者的5年生存率低于高表达者,术后生存时间短于高表达者(P<0.05)。结论胃癌组织中miR-133a-3p和miR-4317均表现为低表达,二者有可能作为胃癌预测和预后判定的潜在标志物,为胃癌患者的预测和预后判定提供一定的参考价值。

Objective To detect the expression of microRNA(miR)-133a-3p and miR-4317 in gastric cancer,and explore the clinical significance of the two.Methods The cancer tissues of 60 gastric cancer patients and their corresponding adjacent tissues were collected.Real-time quantitative PCR(RT-qPCR)was used to detect the expression levels of miR-133a-3p and miR-4317 in tissue samples.The relationship between the expression of miR-133a-3p and miR-4317 and clinical characteristics of patients were also analyzed.Kaplan-Meier survival curve was used to analyze the relationship between the expression of miR-133a-3p and miR-4317 and the prognosis of patients.Results The RT-qPCR results showed that the relative expressions of miR-133a-3p and miR-4317 in gastric cancer tissues were lower than those in adjacent normal tissues,the differences were statistically significant(P<0.05).The expression of miR-133a-3p in gastric cancer tissues were correlated with tumor TNM stage and lymph node metastasis(P<0.05).The expression level of miR-4317 were correlated with lymph node metastasis,distant metastasis and tumor TNM stage(P<0.05).The Kaplan-Meier survival curve showed that the 5-year survival rate of patients with high expression of miR-133a-3p was higher than that of patients with low expression,and the survival time was longer than that of patients with low expression(P<0.05).The 5-year survival rate of patients with low expression of miRNA-4317 was lower than that of patients with high expression of miR-4317,and the survival time was shorter than that of patients with high expression(P<0.05).Conclusion The expression of miR-133a-3p and miR-4317 in gastric cancer tissues is low expression.The expression levels of both miR-133a-3p and miR-4317 can be used as potential markers for prognosis and diagnosis of gastric cancer,and they can provide a reference value for the prognosis and diagnosis of gastric cancer patients.