丙肝患者CCL2基因多态性与白细胞计数的相关性研究

CCL2 Polymorphisms and White Blood Cell Count in Cases with HCV

目的研究丙型肝炎(HCV)患者CC趋化因子配体2(CCL2)单核苷酸多态性(SNP)位点rs1024611、rs1024610、rs2857656、rs13900多态性与白细胞计数的相关性。方法选取2015年4月至12月就诊于大连第六人民医院的245名HCV感染患者,进行外周血白细胞计数,使用TaqMan探针Real-time PCR技术对其进行CCL2 rs1024611、rs1024610、rs2857656、rs13900多态性检测并构建单倍型。在不同遗传模型及单倍型条件下,比较白细胞并细分为单核细胞、淋巴细胞、中性粒细胞、嗜碱性粒细胞、嗜酸性粒细胞计数是否存在统计学差异。结果分别在不同遗传模型条件下比较HCV患者WBC计数的差异,差异无统计学意义(P>0.05)。在245名HCV患者中存在GACT、AAGC、ATGC及GTCT共4种单倍型,所占比例分别为58.37%、33.27%、8.16%及0.20%。各单倍型对HCV患者WBC计数的影响无统计学意义(P>0.05)。结论在HCV患者中CCL2 rs1024611、rs1024610、rs2857656、rs13900多态性并不能影响血液中白细胞计数。

Objective To investigate the correlation of CCL2 single nucleotide polymorphism( SNP)( rs1024611,rs1024610,rs2857656 and rs13900) with white blood cell( WBC) count in HCV cases. Methods 245 HCV cases during the period from April to November in 2015 were enrolled;the peripheral WBC count of each case was collected;the 4 SNP were detected by TaqMan probes with Real-time PCR technique and SNP haplotype was constructed;observation was made to the possible statistical differences in WBC counts( including monocyte,lymphocyte,neutrophil granulocyte,basophil and eosinophil) in different genetic models and in haplotype models. Results There were no significant differences in WBC counts among different genetic models( P>0. 05);4 haplotypes,including GACT( 58. 4%),AAGC( 33. 3%),ATGC( 8. 2%) and GTCT( 0. 2%),were observed in 245 HCV cases;there were no significant differences in WBC counts among different haplotypes( P>0. 05). Conclusions The 4 CCL2 SNPs( rs1024611,rs1024610,rs2857656 and rs13900) are of no significant effect on WBC counts in HCV cases.