新型冠状病毒上海株(nCoV-SH01)的分离和鉴定

Isolation of a 2019 novel coronavirus strain from a coronavirus disease 2019 patient in Shanghai

新型冠状病毒肺炎(coronavirus disease 2019,COVID-19)疫情对人类生命健康构成极大威胁。病毒的分离是构建新型冠状病毒(2019 novel coronavirus,2019-nCoV)细胞感染模型和动物感染模型的基础。本研究利用冠状病毒易感的Vero E6细胞,从1例上海感染者的咽拭子中分离到一株2019-nCoV,命名为nCoV-SH01。对该毒株全基因组采用一代Sanger和二代Illumina法测序,发现该毒株与GenBank MN908947的同源性>99.99%。免疫荧光检测显示,该毒株与COVID-19康复者的血清呈阳性反应。当nCoV-SH01感染Vero E6细胞后,导致典型的合胞体病变,细胞病变效应明显且进展迅速,提示nCoV-SH01可用于进一步建立2019-nCoV的细胞感染和动物感染模型,为开展致病性研究以及抗病毒药物和疫苗研发奠定了基础。

The spread of 2019 novel coronavirus(2019-nCoV)poses a serious threat to human health.We report here the isolation of a 2019-nCoV strain named nCoV-SH01 from the throat swab from a COVID-19 patient in Shanghai.The isolate grows well on Vero E6 cells with typical syncytial cytopathic effects.The nCoV-SH01 genome has been sequenced using sanger sequencing and Illumina sequencing and analyzed,which shares more than 99.99%identity to a reference sequence(GenBank MN9886682019-nCoV Wuhan-Hu-1 isolate)with a difference of only three nucleotides.Immunofluorescence results showed that nCoV-SH01-infected cells reacted with the serum of a convalescent COVID-19 patient,suggesting virus replication in cultured cells.The isolation of nCoV-SH01 provides the basis on which cell and animal infection models would be established to facilitate the study of 2019-nCoV pathogenicity and the development of antiviral drugs and vaccines.