摘要
文章研究了丁香油对鳊(Parabramis pekinensis)保活运输的影响。在单因素试验的基础上通过正交试验确定了最佳保活运输条件。并检测和分析了在模拟运输试验中水质指标和鱼体生化指标的影响。结果显示,最佳保活运输条件为麻醉液浓度15 mg/L、水温9℃和鱼水质量比1﹕3时,保活时间最长达50h,且鱼体成活率为100%。在水体指标中,pH和溶氧量无显著变化,麻醉组氨氮在48h内由0.049增加至4.034 mg/L,水中微生物在24h内显著增加,氨氮的增加和微生物的生长会影响鱼体的保活。在鱼体生化指标中,麻醉组肌肉中糖元含量24h内显著下降,乳酸含量先升后降,pH先降再升,说明在保活运输中糖元消耗产生乳酸;在鱼体血液指标中,麻醉组谷草转氨酶(AST)活性,乳酸脱氢酶(LDH)活性,尿素和肌酐(CREA)在12h后有所上升,血糖(GLU)在36h内显著增加(P<0.05),然后降低;说明随着保活时间的延长鱼体肝脏和肾脏组织代谢水平受到一定的影响。研究表明,在鳊麻醉保活过程中,适当使用丁香油麻醉剂,有助于延长其保活时间和提高存活率。
The effect of clove oil on the live transportation of bream was studied in this paper.Based on a single factor experiment,the optimum conditions for preservation during transportation was determined by orthogonal experiments,and the changes in water quality indicators and their effects on fish biochemical indicators during live transportation were detected and analyzed.The results showed that the optimum conditions for preservation and transportation were an anesthetic solution concentration of 15 mg/L,a water temperature of 9°C and a fish-water ratio of 1﹕3.The survival time was extended by up to 50h,and the fish survival rate was 100%.In terms of the water body indexes,there were no significant changes in pH and dissolved oxygen.The ammonia nitrogen level in the anesthesia group increased from 0.049 mg/L to 4.034 mg/L within 48h.The number of microorganisms in the water increased significantly within 24h.The increase in ammonia nitrogen and the growth of microorganisms affected the survival of fish.In terms of the biochemical indexes of the fish body,the glycogen content in the muscles of the anesthesia group fish decreased significantly within 24h.The content of lactic acid increased significantly and then decreased,and the pH decreased first and then increased,indicating that glycogen consumption produced lactic acid during the transportation of live fish.Among the blood indexes of the fish body,AST activity,LDH activity,urea and creatinine(CREA)increased after 12h and increased significantly after 36h(P<0.05),and then they were decreased in the anaesthetic group.The results showed that the metabolic levels of liver and kidney tissue were affected by the prolongation of the survival time.
作者
丁亚涛
杨丰
王林林
施文正
汪之和
DING Ya-Tao;YANG Feng;WANG Lin-Lin;SHI Wen-Zheng;WANG Zhi-He(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;National R&D Branch Center for Freshwater Aquatic Products Processing Technology(Shanghai),Shanghai 201306,China;Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation,Shanghai 201306,China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2020年第2期407-414,共8页
Acta Hydrobiologica Sinica
基金
国家重点研发计划(2018YFD0901003)资助。
关键词
鳊
丁香油
麻醉
水质
保活
Clove oil
Bream
Anesthesia
Water parameters
Keep-alive