转EPSPS+PAT基因大豆向非转基因大豆的基因漂移研究

Research on the Gene Flow From Transgenic EPSPS+PAT Soybean S4003.14 to Non-transgenic Soybeans.

基因漂移是转基因作物环境风险的重要内容之一。我国不仅是世界上栽培大豆的种植大国和野生大豆最重要的分布区,而且栽培大豆的种植区域与野生大豆的分布区高度重叠,因此,如果转基因大豆在我国进行商业化种植,必须对其向栽培大豆和野生大豆的基因漂移进行严格的评价和研究。以我国自主研发的转EPSPS+PAT基因大豆S4003.14为材料,在吉林省伊通县大田条件下研究其向5种非转基因栽培大豆和5个野生大豆材料的基因漂移。结果表明,S4003.14与5种非转基因栽培大豆和5个野生大豆材料的花期重叠时间分别为17~27和19~23 d;在隔行种植条件下,S4003.14向5种非转基因栽培大豆和5个野生大豆材料基因漂移的异交率分别为0.16%~0.93%和0.06%~0.19%,且杂交后代育性正常;在S4003.14大豆与非转基因栽培大豆之间的距离超过1 m的条件下,未检测到基因漂移的发生。

Gene flow is one of the important environmental risks of transgenic crops.China is one of the most important country with cultivated soybeans and wild soybeans.Furthermore,the distribution area of cultivated soybean and that of wild soybean highly overlap in China.Therefore,strict evaluation and researches on gene flow from transgenic soybean to cultivated soybeans and wild soybeans must be conducted before the commercial cultivation of transgenic soybeans in China.In Yitong County,Jilin Province,transgenic EPSPS+PAT soybean S4003.14,developed by local organization,was studied on its gene flow to 5 cultivated soybeans and 5 wild soybeans.The results show that the flowering overlaps of S4003.14 and the 5 non-transgenic soybeans were 17-27 days,and that of S4003.14 and 5 wild soybean lines were 19-23 days.Under the intercropping condition,as the results of gene flow,the outcrossing rates of transgenic EPSPS+PAT soybean S4003.14 to the 5 non-transgenic soybeans and to the 5 wild soybeans were 0.16%-0.93%and 0.06%-0.19%,respectively.The fertility of the hybrid offsprings was normal.No gene flow was detected when the distance between S4003.14 soybean and non-transgenic cultivated soybeans was more than 1 m.