蛋用鹌鹑IGF-1R基因的多态性与体尺性状相关性分析

Analysis of correlation between polymorphism of IGF-1R gene and body size traits in egg quail

类胰岛素生长因子1型受体(IGF-1R)基因是类胰岛素生长因子(IGFs)在生物体内发挥作用的主要效应器,对机体生长发育进程中有重要影响。为探讨IGF-1R基因多态性与蛋用鹌鹑体尺性状的相关性,采用PCR产物直接测序方法对中国黄羽鹌鹑、北京白羽鹌鹑、朝鲜鹌鹑3种蛋用鹌鹑的IGF-1R基因进行多态性分析,并对IGF-1R基因的多态性与鹌鹑体尺性状进行相关性分析。结果表明,在3个蛋用鹌鹑群体中检测到IGF-1R基因A57G和A72T两个突变位点,A57G位点发生了A/G碱基突变,有AA、AG和GG三种基因型;A72T位点发生了A/T碱基突变,有AA、AT和TT三种基因型。中国黄羽鹌鹑的A57G位点对胫长、胸骨长和胫围有显著影响(P<0.05),A72T位点对体质量和胸骨长有显著影响(P<0.05),组合基因型AGAA对体质量有显著影响(P<0.05),组合基因型AAAA对胫长、胸骨长、胫围有明显影响(P<0.05)。北京白羽鹌鹑的A57G位点对体质量、胫长、体长、胫围有显著影响(P<0.05),A72T位点对胸骨长有显著影响(P<0.05),组合基因型AAAA对体质量和胸骨长有显著影响(P<0.05),组合基因型AAAT、GGAA、GGAT对胫长有显著影响(P<0.05),组合基因型GGAA对胸深有显著影响(P<0.05)。朝鲜鹌鹑的A57G位点对体质量有显著影响(P<0.05),A72T位点对胸骨长和胫围有显著影响(P<0.05),组合基因型AGAA对体质量有显著影响(P<0.05),组合基因型AGAT对胫长和胸宽有显著影响(P<0.05)。因此,IGF-1R基因可以作为候选基因应用于蛋用鹌鹑体尺性状的分子标记辅助选择。

Type 1 insulin-like growth factor receptor(IGF-1R)gene is the main effector of insulin-like growth factor(IGFs).It has important influence on the growth and development of the body.To investigate the correlation within IGF-1R and body size traits of egg quails,the polymorphism of IGF-1R gene was analyzed by direct sequencing of PCR products in Chinese yellow quail,Beijing white quail and Korean quail,and the correlation within the polymorphism of IGF-1R gene and body size traits of quail was analyzed.The results showed that two mutation sites of IGF-1R gene A57G and A72T were detected in three egg quail populations.A/G base mutation occurred at A57G site,including three genotypes of AA,AG and GG.A/T base mutation occurred at A72T site,including three genotypes of AA,AT and TT.The A57G locus of Chinese yellow quail had significant(P<0.05)effects on tibia length,sternal length and tibia circumference,A72T locus had significant(P<0.05)effects on body weight and sternal length,combination genotype AGAA had significant(P<0.05)effects on body weight,combination genotype AAAA had significant effects on tibia length,sternal length and tibia circumference(P<0.05).The A57G locus of Beijing white quail had significant effects on body weight,tibia length,body length and tibia circumference(P<0.05),A72T locus had significant(P<0.05)effect on sternal length,combination genotypes AAAA had significant(P<0.05)effects on body weight and sternal length,and combination genotypes AAAT,GGAA and GGAT had significant(P<0.05)effects on tibia length,the combined genotype GGAA had significant(P<0.05)effect on chest depth.The A57G locus of Korean quail had significant(P<0.05)effect on body weight,and the A72T locus had significant(P<0.05)effect on sternal length and tibia circumference,the combined genotype AGAA had significant(P<0.05)effect on body weight,and the combined genotype AGAT had significant(P<0.05)effect on tibia length and chest width.Therefore,the IGF-1R gene could be used as a candidate gene for molecular marker-assisted selection of body size traits in egg quails.