摘要
为探索外源脯氨酸(Pro)对番茄残留农药降解代谢的调控作用,以番茄浙杂205为供试植物,以杀菌剂百菌清为供试农药,研究外源Pro对番茄体内喷施百菌清后的农药残留量、活性氧含量、抗氧化酶和解毒酶的影响。研究表明,外源3 mmol·L^-1的Pro预处理显著降低了百菌清处理7 d后番茄体内的农药残留量。与对照相比,外源Pro预处理可显著提高百菌清处理后番茄过氧化物酶(POD)、超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDAR)的活性,进一步提高农药处理后12~96 h的GSH/GSSG比值,及谷胱甘肽还原酶(GR)、谷胱甘肽S-转移酶(GST)活性和非蛋白结合态巯基化合物(NPT)含量。因此,外源喷施Pro可提高SOD、MDAR、CAT、POD等抗氧化酶活性,促进GSH的再生,提高GST等解毒酶活性,从而加速番茄体内百菌清的降解代谢,为促进蔬菜残留农药的降解提供一条新途径。
In order to explore the regulation of exogenous proline(Pro)on degradation of residual pesticide in tomato,Zheza 205 was taken as the tested plant and the fungicide chlorothalonil was applied to study the effect of exogenous Pro on pesticide residues.The content of reactive oxygen species(ROS),and the activity of antioxidant enzymes and detoxification enzymes after the application of chlorothalonil were investigated.The results showed that pretreatment of Pro(3 mmol·L-1)reduced the pesticide residue in tomato significantly after 7 days of chlorothalonil application.Compared with the control,exogenous Pro pretreatment could induce the activity of tomato peroxidase(POD),superoxide dismutase(SOD),ascorbate peroxidase(APX),monodehydroascorbic acid reductase(MDAR)significantly after the treatment of chlorothalonil,and increase the GSH/GSSG ratio during the 12-96 h period after pesticide treatment,as well as the activity of glutathione reductase(GR),glutathione S-transferase(GST),and the content of non protein bound thiols(NPT).Therefore,pretreatment of Pro could induce the activity of antioxidant enzymes and GST detoxification enzyme and improve the regeneration of GSH,and thus promote the degradation and metabolism of chlorothalonil in tomato.This study provided a new way to promote the degradation of pesticide residue in vegetables.
作者
刘新宇
陈鹏
张光辉
赵俊杰
李博浩
张南
孙韦珂
许娟娟
叶行涛
魏金鹏
于高波
LIU Xinyu;CHEN Peng;ZHANG Guanghui;ZHAO Junjie;LI Bohao;ZHANG Nan;SUN Weike;XU Juanjuan;YE Xingtao;WEI Jinpeng;YU Gaobo(Heilongjiang Bayi Agricultural University,Daqing 163319,China;Agricultural College,Guangxi University,Nanning 530001,China)
出处
《浙江农业学报》
CSCD
北大核心
2020年第3期437-446,共10页
Acta Agriculturae Zhejiangensis
基金
2018年大学生创新创业训练计划(201810223001)
黑龙江省青年科学基金(QC2018023)
黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2016097)
黑龙江省留学回国人员择优资助项目。
关键词
番茄
脯氨酸
百菌清
抗氧化酶
解毒酶
tomato
proline
chlorothalonil
antioxidant enzyme
detoxification enzyme