心律失常家兔前臂手三阴经微循环变化的比较研究

Microcirculation changes of three yin-meridians of hand in the forearm in rabbits with arrhythmia: a comparative study

目的:观察心律失常家兔前臂手三阴经表面微循环的变化,比较手三阴经经脉反应的差异性。方法:将20只新西兰兔随机分为生理盐水组及模型组,每组10只。经耳缘静脉注射20%氨基甲酸乙酯3.3 m L/kg麻醉,生理盐水组注射1 m L/kg的0.9%氯化钠溶液,模型组注射1 m L/kg的0.4%氯化钡溶液以复制心律失常模型。通过激光散斑血流呈像技术观察分析两组家兔干预前后手三阴经微循环的变化规律。结果:模型组家兔手三阴经微循环灌注量均较造模前明显降低(P<0.01),且手厥阴经降低幅度大于手少阴经(P<0.05),手太阴经与手少阴经比较差异无统计学意义(P>0.05)。手三阴经上观测点微循环变化量与其位置呈负相关关系(P<0.001),即越靠近肘部的观测点微循环灌注量减少的越多。结论:家兔心律失常时,前臂内侧面手三阴经气血厥逆,以手厥阴经最为明显,且手三阴经经脉反应越靠近肘部越明显。

Objective To observe the microcirculation changes of three yin-meridians of hand in the forearm in rabbits with arrhythmia, and to compare the differences of responses among the three yin-meridians of hand. Methods A total of20 New Zealand rabbits were randomly divided into a saline group and a model group, 10 rabbits in each one. The rabbits were anesthetized with intravenous injection of 20% ethyl carbamate(3.3 m L/kg) at ear vein;the saline group was injected with 0.9% sodium chloride solution(1 m L/kg), while the model group was injected with 0.4% barium chloride solution(1 m L/kg) to establish the arrhythmia model. The microcirculation changes of the three yin-meridians of hand were observed and analyzed by laser speckle blood flow imaging technique before and after the intervention. Results Compared before modeling, the microcirculation perfusion of the three yin-meridians of hand in the model group was significantly reduced(P<0.01), and the reduction in the jueyin meridian was more significant than that in the shaoyin meridian(P<0.05), but there was no significant difference between the taiyin meridian and shaoyin meridian(P>0.05). There was a negative correlation between microcirculation perfusion and position(P<0.001), indicating the closer to the elbow, the more the microcirculation perfusion decreased. Conclusion During arrhythmia in rabbits, the dysfunction of qi and blood existes in the three yin-meridians of hand in the forearm, particularly the jueyin meridian. In addition, the closer to the elbow, the more obvious the reaction of the three yin-meridians of hand is.