CD16a对U937细胞联合西妥昔单抗杀伤结直肠癌细胞作用的影响

Effect of CD16a expression in U937 monocyte on cetuximab on cell growth in colorectal cancer

目的探讨CD16a表达变化对U937细胞联合西妥昔单抗杀伤结直肠癌细胞作用的影响。方法将CD16a基因通过慢病毒转染的方法导入U937细胞中,通过检测结肠癌细胞的增殖情况,凋亡情况,细胞周期,迁移、侵袭能力的变化来评价CD16a基因过表达后的U937细胞对结直肠癌细胞杀伤力的影响。结果西妥昔单抗+CD16a过表达U937细胞组,西妥昔单抗+U937细胞组,西妥昔单抗组,U937细胞组,CD16a过表达U937细胞组,空白对照组乳酸脱氢酶(LDH)活性分别为(159.28±7.92),(126.69±17.62),(120.23±5.67),(124.10±16.44),(123.30±6.86)U/L和(95.90±11.44)U/L,西妥昔单抗+CD16a过表达U937细胞组能明显影响结直肠癌细胞的增殖;各组肿瘤细胞凋亡率分别为90.60%±3.57%,74.00%±7.51%,68.10%±8.03%,73.50%±30.67%,68.10%±8.02%和46.20%±17.27%,西妥昔单抗+CD16a过表达U937细胞组诱导肿瘤细胞凋亡率明显高于其余各组;CD16a过表达U937细胞组能够抑制体外培养结直肠癌细胞的侵袭能力。结论CD16a过表达的U937细胞能够增强西妥昔单抗对结直肠癌细胞的杀伤作用,能够抑制体外培养结直肠癌细胞的侵袭能力。

Objective To investigate the effect of CD16a expression changes on monocytes combined with cetuximab in colorectal cancer cells.Methods CD16a gene was imported into U937 monocyte by means of lentivirus infection.The effect of CD16a gene overexpression on the kill ability of U937 monocytes on colorectal cancer cells was evaluated by detecting the tumor proliferation,apoptosis,cell cycle,migration and invasice ability of colorectal cancer cells.Results The activities of LDH in the cetuximab+CD16a overexpression cells group,cetuximab+U937 cells group,cetuximab group,U937 cells group,CD16aoverexpression U937 cells group and blank control group were 159.28±7.92,126.69±17.62,120.23±5.67,124.10±16.44,123.30±6.86 and 95.90±11.44 respectivily.U937 cells with cetuximab+CD16a overexpression significantly inhibited the proliferation of colorectal cancer cells,which showed the statistical difference compared with the other 4 groups.The tumor cell apoptosis rates in various groups were 90.60%±3.57%,74.00%±7.51%,68.10%±8.03%,73.50%±30.67%,68.10%±8.02%and 46.20%±17.27%respectivily.The tumor cell apoptosis induced by U937 cells group with cetuximab+CD16a overexpression was significantly higher that in the other groups.U937 cells with CD16a overexpression could inhibit the invasive ability of colorectal cancer cells in vitro.Conclusion U937 monocytes with CD16a overexpression can enhance the killing effect of cetuximab on colon cancer cells,and can inhibit the invasive ability of colon cancer cells cultured in vitro.