肝脓肿肺炎克雷伯菌血清型及毒力基因检测

Serotype and virulence gene detection of Klebsiella pneumoniae in liver abscess

目的探讨肝脓肿肺炎克雷伯菌的血清型及毒力基因携带情况。方法收集2018年4月至2019年4月该院肝脓肿住院患者标本分离的肺炎克雷伯菌菌株32株;所有菌株进行拉丝试验,用聚合酶链反应(PCR)法检测肺炎克雷伯菌的主要血清型及毒力基因的分布情况。结果32株非重复肺炎克雷伯菌中高黏液型26株(81.25%),血清型K1型18株(56.25%),K2型7株(21.88%),K5型1株(3.13%);这3种血清型均携带有毒力基因rmpA+aero+uge。结论肝脓肿高毒力肺炎克雷伯菌以K1、K2血清型为主,并携带了大量的毒力基因,多见于糖尿病老年患者,对临床常用的抗生素敏感性高。

Objective To investigate the serotype and virulence gene carrying situation of Klebsiella pneumoniae in liver abscess.Methods A total of 32 strains of Klebsiella pneumoniae isolated from hospitalized patients with liver abscess in this hospital from April 2018 to April 2019 were collected.All strains were subjected to a wire drawing test.The main serotypes of Klebsiella pneumoniae strains and the distribution situation of virulence genes were detected by PCR.Results Among 32 strains of non-repetitive Klebsiella pneumoniae,the high mucus type accounted for 26(81.25%),the K1 serotype had 18 strains(56.25%),and the K2 serotype had 7 strains(21.88%),K5 serotype had 1 strains(3.13%);all three serotypes carried the virulence gene rmpA+aero+uge.Conclusion Highly virulent Klebsiella pneumoniae in liver abscess is mainly dominated by K1 and K2 serotypes,whic carry a large number of virulence genes.This disease is more common in elderly people with diabetes and has high sensitivity to commonly used antibiotics in clinic.