威灵仙中5-HMF含量测定及其来源研究

Determination and Production Mechanism of 5-HMF in Clematidis Radix Using HPLC Technique

为威灵仙的提取方法改进提供研究依据,优化建立威灵仙中5-HMF的HPLC分析方法并进行方法学验证,在此基础上,考察提取条件对威灵仙及其不同部位中5-HMF含量的影响。结果表明:威灵仙药材中的还原型寡糖在煎煮过程中会降解产生5-HMF,提取温度是影响5-HMF含量的关键因素。HPLC测定5-HMF在0.3508~21.0530μg·mL^-1范围内线性关系良好(r=0.9999),平均加样回收率为99.8%,RSD为1.2%。不同产地、基原的威灵仙经煎煮后的5-HMF含量差异较大,介于0.0148~0.2116 mg·g^-1,棉团铁线莲含量高于威灵仙和东北铁线莲。建立的HPLC分析方法操作简单、结果准确、重现性好,可用于威灵仙中5-HMF的含量测定,研究结果为改进威灵仙提取方法、提升临床用药安全性提供了科学依据。

This paper is to provide the research basis for improving the extraction method of Clematidis Radix.The HPLC analysis method of 5-HMF in Clematidis Radix was optimized and the methodology was verified.On this basis,the effects of extraction technology on the content of 5-HMF in Clematidis Radix and its different parts were investigated.The results showed that 5-HMF was mainly produced by the reductive oligosaccharide in Clematidis Radix,and the extraction temperature was the key factor affecting the content of 5-HMF.The linear range of 5-HMF was in the range of 0.3508-21.0530μg·mL^-1(r=0.9999).The recovery rate of this method was 99.8%with the RSD of 1.2%.The contents of 5-HMF varied greatly in Clematidis Radix from different producing areas and the range is 0.0148-0.2116 mg·g^-1.The content of Clematidis Radix hexapetala was higher than that of Clematidis Radix chinensis and Clematidis Radix manshurica.The method of the assay for 5-HMF using HPLC is simple,accurate and reproducible,and it can be used for the determination of 5-HMF in Clematidis Radix.The results can provide scientific evidence for optimizing the extraction method and improving the safety of clinical medication of Clematidis Radix.