短乳杆菌AR247的抗氧化成分及其抗衰老作用

Antioxidant Compounds of Lactobacillus brevis AR247 and Its Anti-aging Effect

对短乳杆菌AR247的抗氧化成分及其对D-半乳糖致衰老小鼠的保护作用进行研究。通过分级萃取等试验得知,短乳杆菌AR247的抗氧化成分多为水溶性组分和胞内组分。通过皮下注射D-半乳糖【150 mg/(kg·d)】6周后,建造衰老模型,然后灌胃6周1.0×10^9 CFU/mL(1 mL/100 g)的短乳杆菌AR247。结束后测定血清、肝脏和脑中的抗氧化水平,如过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和谷胱甘肽(GSH)、丙二醛(MDA)的含量。同时测定小鼠血清中的白介素2(IL-2)、肿瘤坏死因子α(TNF-α)含量。结果表明:短乳杆菌AR247能延缓D-半乳糖引起的衰老,如恢复体重和脑指数至正常水平,使小鼠的CAT、SOD、GSH-Px活性升高(P<0.05),GSH含量升高(P<0.05),MDA含量降低(P<0.05),其中肝脏MDA含量降至正常的66.13%。与模型组相比,免疫水平提高(P<0.05),IL-2含量提高至正常组的104.50%。短乳杆菌AR247具有抗氧化和延缓衰老作用,其体内抗氧化的作用机制可能与改善机体的抗氧化功能和增强免疫功能有关。

In this study,the antioxidant ingredients of Lactobacillus brevis AR247 were explored,then anti-aging effects of L.brevis AR247 on D-galactose induced aging mice were measured.The aqueous phase and intracellular constituents of L.brevis AR247 showed higher antioxidant activity than others.The aging mice model was established by injecting of D-galactose on the concentration of 150 mg/(kg·d)for 6 weeks.These mice were simultaneously administrated by gavage with L.brevis AR247 on the concentration of 1.0×10^9 CFU/mL(1 mL/100 g).After for 6 weeks,the activities of catalase(CAT),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),and the contents of glutathione(GSH)and malondialdehyde(MDA)in serum,liver and brain were measured by commercial reagent kits.The contents of Interleukin 2(IL-2)and tumour necrosis factor-α(TNF-α)in serum were also determined.The results showed that L.brevis AR247 had antagonism effects on D-galactose-induced aging mice such as recovered body weight and brain index to normal levels,and it could increase the activities of CAT,SOD and GSH-Px(P<0.05),the content of GSH(P<0.05),and reduce MDA levels(P<0.05)such as reduce the content of MDA in liver to 66.16%of normal group.Compared with the aging model group,the content of serum IL-2 in L.brevis AR247 group was significantly increased(P<0.05),and it increased to 104.50%of normal group.In conclusion,L.brevis AR247 showed antioxidant and anti-aging effects.The underlying mechanism might be related to improvement of antioxidant function and immune function in the body.