鲣鱼蛋白肽谱效关系研究

Studies on the Correction of Spectrum and Activities of Protein Hydrolysates of Bonito

采用鲣鱼为原料,以蛋白质回收率为指标,酶解制备不同工艺条件的样品。通过测定其黄嘌呤氧化酶(XOD)抑制活性、ORAC值、分子质量分布图谱和总氨基酸含量,构建鲣鱼蛋白肽体外化学活性与其分子质量分布图谱、总氨基酸含量图谱之间的谱效关系。结合超高效液相-质谱联用(LC-MS/MS)方法研究其降尿酸肽和抗氧化肽的分布。研究表明,鲣鱼蛋白肽的XOD抑制活性与其ORAC值具有显著的相关性。由多肽分子质量<1ku所占含量的分布区段和脂肪族氨基酸的含量可初步预测鲣鱼蛋白肽的化学活性。质谱分析表明,纯化组分的质谱基峰图在不同时间段的信号强度与鲣鱼蛋白肽的降尿酸活性和抗氧化活性具有明显的相关性。

Eight bonito protein hydrolysates were prepared under different enzymatic hydrolysis processing with the index of protein recovery rate and bonito fish as raw materials.The xanthine oxidase(XOD)inhibitory activity,ORAC values,molecular weight distribution and the total contents of amino acid was measured.The correlation of XOD inhibitory activity and antioxidant activity of bonito protein hydrolysates with molecular weight distribution and the total contents of amino acid was constructed.The ultra-performance liquid chromatography-high resolution mass spectrometry(LC-MS/MS)was used to study the distribution of those peptides with XOD inhibitory activity and antioxidant activity.The results demonstrated that the correlation between XOD inhibitory activity and ORAC values was significant.The distribution of those peptides molecular weight<1 ku and the contents of aliphatic amino acids could be used to preliminarily evaluate the activities of bonito protein hydrolysates.After purification,the concentration of active peptides of fractions isolated from bonito protein hydrolysates was enhanced,and the total ion flow diagram of the purified fractions was related to the their uric-acid-lowering activity and antioxidant activity.