赣南脐橙代谢组学研究

Studies on Metabonomics of Gannan Navel Orange

采用气相色谱-质谱联用技术测定赣南和其它地区的脐橙挥发性物质,对不同产地脐橙进行代谢组学研究,为赣南脐橙品质保障提供科学依据。通过GC-MS分析脐橙的挥发性成分,经数据库匹配结合相关文献鉴定物质,采用正交偏最小二乘法-判别分析(OPLS-DA)的s-plot图并结合对各组分的贡献率(VIP>1,P<0.05)筛选潜在生物标志物。结果表明,从赣南脐橙中共鉴定出66种挥发性成分,分别为萜烯类、醛类、醇类、酯类、酮类等,其中含量最高的为D-柠檬烯。根据OPLS-DA分析筛选出5个潜在生物标志物,即(1S)-(+)-3-蒈烯、4-蒈烯、壬醛、(Z)-石竹烯、桉烷-3,7(11)-二烯,其可以作为区分赣南脐橙和其它地区脐橙的潜在生物标志物。

Volatile compounds of navel orange in Gannan and other areas by gas chromatography-mass spectrometry(GC-MS),and the metabolomics of navel orange in different areas was studied,which provided scientific basis for quality assurance of Gannan navel orange.The volatile components of navel orange were analyzed by GC-MS and identified by database matching with the relevant reports.The potential biomarkers were screened by the s-plot of orthogonal partial least squares discriminant analysis(OPLS-DA)and the variable importance in the projection value(VIP>1,P<0.05).The results showed that 66 volatile components were identified in Gannan navel orange,which were terpenes,aldehydes,alcohols,esters and ketones,the highest content was D-limonene.The five volatile components were(1S)-(+)-3-Carene,4-Carene,nonanal,(Z)-Caryophyllene,Eudesma-3,7(11)-diene,which can serve as potential biomarkers for the distinction between navel orange and other regions of the navel orange.