摘要
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9)是一种新型基因编辑系统,是由细菌和古细菌对抗入侵病毒及外源DNA的适应性免疫防御系统演变而来的。该系统具有简易、精准、经济和高效的优点,目前已广泛应用于基因编辑及其相关研究中。本文总结了近几年CRISPR/Cas9技术在猪基因组编辑中的研究进展,并展望了其应用前景。
CRISPR/Cas9(clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9)is a novel editing system,which is originally developed from the bacteria defense immune mechanism against viruses infection and plasmid transfer.As a simple,accurate,economical and efficient tool,this technology has been extensively used in the gene-editing and related research fields.The progress and application of CRISPR/Cas9 technology in pig genome editing in the past few years are summarized in this review,and also discusses its future perspectives in pig breeding and clinical trials.
作者
刘思远
卢丹
唐中林
LIU Siyuan;LU Dan;TANG Zhonglin(Genome Analysis Laboratory of Ministry of Agriculture,Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China;Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Hunan Agricultural University,Changsha 410128,China;Yunnan Agricultural University,Kunming 650201,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2020年第3期409-416,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家转基因重大专项(2016ZX08006002-005)
深圳市技术攻关重点项目(JSGG20180507182028625)。
