2例犬肝胆管细胞癌的病理学诊断

Pathological Diagnosis of Two Cases of Canine Cholangiocellular Carcinoma

肝胆管细胞癌是来源于肝胆管上皮细胞的恶性肿瘤,在多物种中都有发生。笔者运用组织病理学和免疫组织化学的方法,对2例犬肝肿瘤进行了诊断。结果显示:肝肿瘤眼观均为圆球形白色肿块,组织病理学检查肿瘤组织由呈腺管状排列的瘤细胞构成,有的腺管中央有坏死脱落的细胞,有的有黏液样物质,腺管间以薄的纤维结缔组织分隔,瘤组织有大片的坏死。瘤细胞多呈卵圆形,细胞核大、卵圆形,核分裂象多见,瘤细胞的CK19阳性表达,而CK18和AFP呈阴性表达。根据组织病理学和免疫组织化学检查结果判断,2例犬肝肿瘤为肝胆管细胞癌,本研究为犬肝肿瘤诊断积累了病理学资料。

Cholangiocellular carcinoma is a malignant tumor derived from hepatic bile duct epithelial cells, which occurs in many species. In this study, two cases of canine liver tumors were diagnosed by histopathology and immunohistochemistry. Macroscopic of liver tumor is a spherical white mass, histopathological examination the tumor tissue consists of tumor cells arranged in a glandular tube, in the center of the gland have some necrotic and exfoliated cells,and some have mucus-like substances, the ducts are separated by thin fibrous connective tissue, and the tumor tissue has large area necrosis. The tumor cells are mostly oval, the nucleus is large, oval, and mitotic image is more common, CK19 was positively expressed in tumor cells, while CK18 and AFP were negatively expressed. According to the results of histopathology and immunohistochemistry, 2 cases of canine liver tumors were Cholangiocellular carcinoma, this study accumulated pathological data for the diagnosis of canine liver tumors.