孟鲁司特钠对支气管肺发育不良大鼠Th1Th2细胞平衡及其相关细胞因子水平的调节作用

Regulating effects of montelukast sodium on Th1Th2 cells balance and related cytokines levels in rats with bronchopulmonary dysplasia

目的:观察孟鲁司特钠(MK)对支气管肺发育不良(BPD)大鼠Th1Th2细胞平衡及其相关细胞因子水平的调节作用。方法:随机选择SPF级健康SD孕3周左右,体质量在200~250 g孕鼠60只进行实验,将60只孕3周左右自然分娩SD母鼠与新生大鼠分为3组,每组20只,分别为:MK处理BPD模型组(MK组)、BPD模型组(BPD组)、常氧饲养对照组(对照组)。MK组、BPD组饲养在95%的高氧环境中,其中MK组给予MK(腹腔注射,10 mg·kg^-1·d^-1)处理,BPD组给予等剂量0.9%氯化钠溶液处理。对照组亦给予等剂量0.9%氯化钠溶液处理,饲养在常氧环境中。比较3组大鼠的肺功能、肺损伤局部巨噬细胞亚群和Th细胞功能亚群、肺损伤局部促炎和抗炎细胞因子水平。结果:大鼠Ri、Re、Th1、Th1/Th2、IFN-γ、TNF-α、IL-1β、IL-6、IL-4、IL-10、IL-13水平BPD组高于MK组,MK组高于对照组(P<0.05);Cl、MVV、FEV1/FVC、Th2水平BPD组低于MK组,MK组低于对照组(P<0.05)。结论:MK具有调节支气管肺发育不良大鼠Th1、Th2细胞平衡,降低相关细胞因子水平,改善肺功能的作用。

Objective:To observe the regulating effects of montelukast sodium(MK)on the balance of Th1Th2 cells and levels of related cytokines in rats with bronchopulmonary dysplasia(BPD).Methods:Sixty healthy SPF SD rats,3 weeks of gestation and weighing 200-250 g,were randomly selected for the experiment,and 60 natural born SD rats and newborn rats were divided into the BPD model treated with MK group(MK group),BPD model group(BPD group)and normoxia feeding control group(control group)(20 rats in each group),respectively.The MK group and BPD group were fed in 95%hyperoxia environment,the MK group was intraperitoneally injected with 10mg·kg^-1·d^-1 of MK,and the BPD group was injected with the same volume of 0.9%sodium chloride solution.The control group was injected with the same volume of 0.9%sodium chloride solution,and fed in the normoxic environment.The lung function,macrophage subsets,Th cell subsets and levels of pro-inflammatory and anti-inflammatory cytokines were compared among three groups.Results:The levels of Ri,Re,Th1,Th1/Th2,IFN-γ,TNF-α,IL-1β,IL-6,IL-4,IL-10 and IL-13 in BPD group were higher than those in MK group and those in MK group were higher than those in control group(P<0.05).The levels of Cl,MVV,FEV1/FVC,Th2 in BPD group were lower than those in MK group,and those in MK group were lower than those in control group(P<0.05).Conclusions:MK can regulate the balance of Th1Th2 cells,and reduce the levels of related cytokines in rats with BPD.