补肾药膳方影响卵巢去势骨质疏松模型大鼠骨形成机制探讨

Effect of reinforcing kidney medicinal diet on bone formation in ovariectomized osteoporotic rats

目的观察骨形成代谢指标BGP的变化,探讨补肾药膳影响卵巢去势骨质疏松模型大鼠骨形成的机制。方法将60只SPF级雌性SD大鼠随机分为6组,各组10只,正常组、假手术组、模型空白组等选用一般饲料饲养,高剂量药膳组、低剂量药膳组、骨疏康颗粒组等造模1周后进行灌胃。3个月后取大鼠血清标本以ELISA法检测血清BGP水平。结果低剂量组与高剂量组与模空组之间差异无统计学意义,但血清BGP具有下降的趋势,骨疏康组血清BGP水平高于模空组(P<0.05)。结论补肾药膳对卵巢去势绝经后骨质疏松大鼠模型的骨形成代谢指标有一定的改善作用,在改善血清BGP方面弱于BALP、PINP等指标,补肾药膳方影响PMOP症机制值得进一步探讨。

Objective To observe the changes of bone formation metabolic index BGP and to explore the mechanism of the effect of kidney-tonifying herbal diet on bone formation in ovariectomized osteoporotic rats.Methods Sixty SPF female SD rats were randomly divided into 6 groups,with 10 in each group.Rats in normal group,sham-operated group,and model blank group were fed with general diet.Rats in high-dose diet group,low-dose diet group,and Gushukang granule group were fed after 1 week of modeling.Three months later,serum samples of the rats were taken to detect the concentration of BGP using ELISA.Results There was no significant difference between low-dose group and high-dose group,but there was a decrease trend in serum BGP.The serum BGP concentration in Gushukang group was higher than that in model group(P<0.05).Conclusion Kidney-tonifying herbal diet improves the bone formation and metabolism of ovariectomized postmenopausal osteoporotic rats.The change of serum BGP is weaker than that of BALP and PINP.The mechanism of kidney-tonifying herbal diet prescription affecting PMOP is worth further exploring.